P. Roy, Rei Abe-Yoshizumi, Yoshitaka Kato, H. Kandori, T. Buckup
{"title":"用时间分辨相干拉曼光谱绘制野生型和突变水龙鱼感觉紫红质的异构化动力学","authors":"P. Roy, Rei Abe-Yoshizumi, Yoshitaka Kato, H. Kandori, T. Buckup","doi":"10.1109/CLEOE-EQEC.2019.8872608","DOIUrl":null,"url":null,"abstract":"The light induced isomerization of retinal protonated Schiff base (RPSB) inside the protein pocket is one of the fastest (<ps) and most stereo-selective photochemical reactions in nature [1]. The ground state structure of the RPSB and its surrounding protein architecture are known to play a central role in this reaction. It has been a longstanding question how each factor individually influences the reaction dynamics. In this context, Anabaena Sensory Rhodopsin (ASR), a recently discovered microbial retinal protein, serves as an ideal system to answer this question as it binds two structural isomers (all-trans and 13-cis) of the RPSB within the same protein constructions in its photocycle. In this work, the photo-isomerization dynamics of the RPSB in ASR has been explored with the help of time resolved coherent Raman techniques like pump-degenerate four-wave-mixing (pump-DFWM) (Fig. 1(a)) and pump-impulsive vibrational spectroscopy (pump-IVS). Both methods report on the structural changes of the RPSB during the photochemical reaction by tracking the transient frequency shifts of the vibrational modes in the excited state.","PeriodicalId":6714,"journal":{"name":"2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)","volume":"57 1","pages":"1-1"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isomerization Dynamics of Wild Type and Mutated Anabaena Sensory Rhodopsin Mapped by Time-Resolved Coherent Raman Spectroscopy\",\"authors\":\"P. Roy, Rei Abe-Yoshizumi, Yoshitaka Kato, H. Kandori, T. Buckup\",\"doi\":\"10.1109/CLEOE-EQEC.2019.8872608\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The light induced isomerization of retinal protonated Schiff base (RPSB) inside the protein pocket is one of the fastest (<ps) and most stereo-selective photochemical reactions in nature [1]. The ground state structure of the RPSB and its surrounding protein architecture are known to play a central role in this reaction. It has been a longstanding question how each factor individually influences the reaction dynamics. In this context, Anabaena Sensory Rhodopsin (ASR), a recently discovered microbial retinal protein, serves as an ideal system to answer this question as it binds two structural isomers (all-trans and 13-cis) of the RPSB within the same protein constructions in its photocycle. In this work, the photo-isomerization dynamics of the RPSB in ASR has been explored with the help of time resolved coherent Raman techniques like pump-degenerate four-wave-mixing (pump-DFWM) (Fig. 1(a)) and pump-impulsive vibrational spectroscopy (pump-IVS). Both methods report on the structural changes of the RPSB during the photochemical reaction by tracking the transient frequency shifts of the vibrational modes in the excited state.\",\"PeriodicalId\":6714,\"journal\":{\"name\":\"2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)\",\"volume\":\"57 1\",\"pages\":\"1-1\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/CLEOE-EQEC.2019.8872608\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/CLEOE-EQEC.2019.8872608","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}