研究前生物、免疫刺激特性及其对生殖功能的影响

Q3 Medicine
Е. П. Анохина, М. М. Исува, С. В. Старцева, Е. А. Мотина, Н. А. Михайлова, Ольга Сергеевна Корнеева
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引用次数: 0

摘要

的目标。探讨饲粮中聚焦对实验性生态失调实验动物胃肠道菌群、非特异性免疫体液因子以及卵母细胞聚焦程度和可受精卵母细胞比例的影响。材料和方法。通过对实验小鼠肠道菌群的分析,在实验生态失调的背景下,研究了病灶的益生元特性。采用酶免疫分析法,根据小鼠免疫后血清抗体形成水平,对小鼠非特异性免疫的影响因素进行研究。通过荧光显微镜观察实验小鼠卵母细胞的发光强度来评估卵母细胞的聚焦程度。结果。在所有测试剂量中使用聚焦导致胃肠道微生物群的组成和数量的恢复。矫正生态失调的最佳聚焦浓度为实验动物体重的0.02%。在实验动物日粮中添加浓度为体重0.008%的焦斑菌,免疫应答水平最高。以小鼠体重的0.008%加入聚焦剂,可提高卵母细胞的聚焦程度和受精率。结论。确定了灶的双歧性和生乳活性。显示了聚焦刺激血清中抗体水平增加的能力。揭示了小鼠饮食中的聚焦对卵母细胞的聚焦程度有积极作用的趋势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ИССЛЕДОВАНИЕ ПРЕБИОТИЧЕСКИХ, ИММУНОСТИМУЛИРУЮЩИХ СВОЙСТВ ФУКОЗЫ И ЕЕ ВЛИЯНИЯ НА РЕПРОДУКТИВНУЮ ФУНКЦИЮ
Aim. Investigation of the effect of fucose in the diet on the gastrointestinal microflora of experimental animals with experimental dysbiosis, the humoral factors of nonspecific immunity, as well as the degree of fucosylation of oocytes and the proportion of oocytes that can be fertilized. Materials and methods. Prebiotic properties of fucose were studied by analyzing the luminal microflora of experimental mice against the background of experimental dysbiosis. Investigation of factors of nonspecific immunity was carried out after immunization of mice according to the level of antibody formation in blood serum by the method of enzyme immunoassay. The degree of fucosylation of oocytes was assessed by the intensity of their luminescence upon microscopy of oocytes of experimental mice on a fluorescent microscope. Results. The use of fucose in all tested doses led to the restoration of the composition and quantity of the gastrointestinal microflora. For the correction of dysbiosis, the optimal concentration of fucose was 0.02% of the body weight of the experimental animals. Inclusion of fucose in a diet of experimental animals in the amount of 0.008% to the body weight provided the highest level of immune response. The degree of fucosylation of oocytes, the proportion of oocytes capable of fertilization was increased when fucose were introduced in the amount of 0.008% to the body weight of the mice. Conclusion. Bifidogenic and lactogenic activity of fucose is established. The ability of fucose to stimulate an increase in the level of antibodies in in blood serum is shown. The tendency of positive effect of fucose in the diet of mice on the degree of fucosylation of oocytes was revealed.
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来源期刊
Zhurnal mikrobiologii, epidemiologii, i immunobiologii
Zhurnal mikrobiologii, epidemiologii, i immunobiologii Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
1.40
自引率
0.00%
发文量
51
审稿时长
8 weeks
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