The Skin Wrinkle Improvement Effect of Mixed Extracts of Bletilla striata Reichenbach fil., Ampelopsis japonica, Atractylodes japonica, etc.

Purpose: The purpose of this study is to evaluate the skin wrinkle improvement effect of a mixture of Bletilla striata Reichenbach fil., Ampelopsis japonica, Angelica dahurica, Atractylodes japonica, Ligusticum sinense Oliv, Ligusticum officinale, and Angelica gigas (BAA).Methods: In this study, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity evaluation, SOD (superoxide dismutase)-like activity, xanthine oxidase inhibitory activity evaluation, anti-wrinkle activity test, hyaluronic acid production confirmation, and cytotoxicity evaluation were performed to evaluate the cosmetic functionality of the fermented mixed extract.Results: As a result of ABTS radical analysis, BAA-4 was the highest at 500 μg/mL (63.7%). As a result of DPPH radical analysis, BAA-4 was the highest at 61.4%. As a result of SOD-like activity analysis, it was found that BAA-4 increased SOD-like activity to 33.0±3.7% at a concentration of 500 μg/mL. As for the xanthine oxidase inhibitory activity analysis result, the same as the SOD-like activity result, BAA-4 showed the highest concentration of 1.3±2.8% at the concentration of 500 μg/mL. As a result of collagenase activity and elastase analysis, BAA-4 was the highest at a concentration of 500 μg/mL (46.1±3.6% and 51.9±3.9%, respectively). As for the hyaluronic acid content, BAA-4 was found to be the highest at 257.4 ng/mL, and cell viability was found to be more than 95% at allconcentrations, confirming that all mixed extracts (BAA-1 to 4) had no cytotoxicity. Conclusion: These research results suggest that the mixed extract has an excellent collagen production promoting effect and can be applied as a safe natural wrinkleimproving cosmetic material that does not cause skin irritation.