电子烟液对口腔共生链球菌群落生物膜形成和生长的机制影响:调味剂的作用

IF 0.5 Q4 DENTISTRY, ORAL SURGERY & MEDICINE
Christina Xu, D. Palazzolo, Giancarlo A. Cuadra
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引用次数: 3

摘要

背景:电子烟已经成为一个全球性的健康问题。随着研究的继续,越来越多的研究开始质疑电子烟调味添加剂的相对安全性。口腔是接触电子液体气溶胶的第一个部位,因此对调查至关重要。由于共生细菌生物膜对口腔健康的重要性,我们试图探索电子烟油±香料对单种和多种生物膜形成和生长的影响,并探讨其抑制机制。方法:采用四种口腔共生菌(戈多氏链球菌、中间链球菌、米氏链球菌和口腔链球菌),对无风味和有风味(烟草、薄荷、肉桂、草莓、蓝莓)电子液体进行定量和共聚焦生物膜分析、死亡曲线和菌落形成单位(CFU)评估。结果:各风味剂对单种和多种生物膜的生长均表现出剂量依赖性的抑制作用。此外,cfu、死亡曲线和光镜显示,调味剂对这些口腔链球菌的生长具有杀菌抑制作用。结论:加味电子烟油比无味电子烟油更不利于口腔共生菌的生物膜形成和生长。因此,电子液体调味剂可能对口腔微环境构成风险,进而对全身健康构成风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mechanistic Effects of E-Liquids on Biofilm Formation and Growth of Oral Commensal Streptococcal Communities: Effect of Flavoring Agents
Abstract Background: Vaping has become a global health concern. As research continues, more studies are beginning to question the relative safety of E-liquid flavoring additives. The oral cavity is the first site of exposure to E-liquid aerosol, making it critical for investigation. Because of the importance of commensal bacterial biofilms for oral health, we sought to explore the effects of E-liquids ± flavors on the formation and growth of single- and multi-species biofilms and to investigate the mechanism of inhibition. Methods: Quantitative and confocal biofilm analysis, death curves, and colony-forming units (CFU) were evaluated with flavorless and flavored (tobacco, menthol, cinnamon, strawberry, blueberry) E-liquids using four strains of oral commensal bacteria (Streptococcus gordonii, Streptococcus intermedius, Streptococcus mitis, and Streptococcus oralis). Results: All flavoring agents show a dose-dependent inhibition in the growth of single-species and multi-species biofilms. Furthermore, CFUs, death curves, and light microscopy show that flavoring agents have a bactericidal mode of inhibition on the growth of these oral streptococci. Conclusions: These results show that flavored, rather than unflavored, E-liquids are more detrimental to biofilm formation and growth of oral commensal bacteria. Consequently, E-liquid flavorings agents could pose risks to the oral microenvironment, and by extension, to systemic health.
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来源期刊
Open Dentistry Journal
Open Dentistry Journal DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
2.00
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发文量
86
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