多激光扫描共聚焦荧光内窥镜亚细胞成像方案(特邀)

IF 6.7 1区计算机科学 Q1 Physics and Astronomy

Progress in Electromagnetics Research-Pier Pub Date : 2020-01-01 DOI:10.2528/pier20092201

Xiaomin Zheng, Xiang Li, Qiaowen Lin, Jiajie Chen, Yueqing Gu, Yonghong Shao

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引用次数: 3

摘要

荧光共聚焦激光扫描内窥镜是一种将共聚焦显微镜和内窥镜结合起来进行体内亚细胞结构成像的新型工具，其分辨率与传统显微镜相当。本文提出了一种基于光纤束和488 nm和650 nm两条激发激光线的三通道荧光共聚焦显微系统。红、绿、蓝三个荧光光电倍增管检测通道可同时记录单个样品部位的多色荧光信号。并验证了其在亚细胞水平上进行体内多通道荧光检测的能力。此外，该系统还实现了直径为154 μm的高分辨率有效视场。它具有多激光扫描、多通道检测、灵活探测和体内成像能力，将成为生物化学研究和诊断的有力工具，如研究纳米药物在小动物体内的转运机制。

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MULTI-LASER SCANNING CONFOCAL FLUORESCENT ENDOSCOPY SCHEME FOR SUBCELLULAR IMAGING (INVITED)

Fluorescence confocal laser scanning endomicroscopy is a novel tool combining confocal microscopy and endoscopy for in-vivo subcellular structure imaging with comparable resolution as the traditional microscope. In this paper, we propose a three-channel fluorescence confocal microscopy system based on fiber bundle and two excitation laser lines of 488 nm and 650 nm. Three fluorescent photomultiplier detecting channels of red, green and blue can record multi-color fluorescence signals from single sample site simultaneously. And its ability for in-vivo multi-channel fluorescence detection at subcellular level is verified. Moreover, the system has achieved an effective field of view of 154 μm in diameter with high resolution. With its multi-laser scanning, multi-channel detection, flexible probing, and in-vivo imaging abilities it will become a powerful tool in bio-chemical research and diagnostics, such as the investigation of the transport mechanism of nano-drugs in small animals.

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来源期刊

Progress in Electromagnetics Research-Pier 物理-电信学

CiteScore

7.20

自引率

3.00%

发文量

审稿时长

1.3 months

期刊介绍： Progress In Electromagnetics Research (PIER) publishes peer-reviewed original and comprehensive articles on all aspects of electromagnetic theory and applications. This is an open access, on-line journal PIER (E-ISSN 1559-8985). It has been first published as a monograph series on Electromagnetic Waves (ISSN 1070-4698) in 1989. It is freely available to all readers via the Internet.