Impact of Nanochitin on Serum Concentration of Iron and Calcium in Wistar Rats

Background and objectives : Considering the increasing use of nanochitin for the removal of heavy metals from aqueous solutions, examining the biological effects of this substance on the level of essential metals for humans and animals is crucial. Therefore, this study investigated impact of oral administration of nanochitin on serum levels of iron (Fe) and calcium (Ca) in Wistar rats. Methods : Twenty male Wistar rats were randomly divided into four treatment groups and one control group. Two groups were fed with nanochitin at doses of 1.6 and 2.6 μg/g for 6 weeks, and the other two groups received the mentioned doses for 10 weeks. Serum concentrations of Fe and Ca were measured using atomic absorption spectroscopy. Results : Oral administration of 2.6 μg/g nanochitin for 10 weeks caused a significant decrease in serum Ca and Fe concentrations ( p <0.05). Oral administration of 1.6 and 2.6 μg/g nanochitin for 6 weeks caused a non-significant reduction in serum Fe and Ca concentrations ( p >0.05). However, nanochitin consumption for 10 weeks resulted in a significant decrease in serum Fe concentration but not Ca. Conclusion : The limited reduction of serum Fe and Ca concentrations after oral consumption of nanochitin at a low dose and for a limited duration indicates that the controlled use of nanochitin could be safe for animals. However, complementary studies are needed to determine the exact effects of nanochitin on the animals’ bodies. On the other hand, it is recommended to use Fe and Ca supplements after consuming high doses of nanochitin for longer periods.