{"title":"溶胶-凝胶型辣根过氧化物酶生物传感器用于化学发光流量测定过氧化氢","authors":"Jun Li, Kemin Wang, Xiaohai Yang, D. Xiao","doi":"10.1039/A901946D","DOIUrl":null,"url":null,"abstract":"A chemiluminescent (CL) H2O2 sensor based on horseradish peroxidase (HRP) immobilized by the sol–gel method has been proposed in this paper. A new process of fabricating a non-cracking HRP encapsulated sol–gel membrane has been suggested. Flow injection analysis (FIA) was used to give reproducible results. Under optimum conditions, the sensor showed a linear response toward hydrogen peroxide in the range 0.01–2 mM with a detection limit of 8 µM. The linear relative coefficient r = 0.998. The sensor showed rapid response, good reproducibility (relative standard deviation = 2.4%) and a long lifetime of at least two months. The proposed sensor was used to determine a 30% H2O2 solution that has been stored for a long time. The results were in good agreement with the standard volumetric method.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"9 1","pages":"195-197"},"PeriodicalIF":0.0000,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"14","resultStr":"{\"title\":\"Sol–gel horseradish peroxidase biosensor for the chemiluminescent flow determination of hydrogen peroxide\",\"authors\":\"Jun Li, Kemin Wang, Xiaohai Yang, D. Xiao\",\"doi\":\"10.1039/A901946D\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A chemiluminescent (CL) H2O2 sensor based on horseradish peroxidase (HRP) immobilized by the sol–gel method has been proposed in this paper. A new process of fabricating a non-cracking HRP encapsulated sol–gel membrane has been suggested. Flow injection analysis (FIA) was used to give reproducible results. Under optimum conditions, the sensor showed a linear response toward hydrogen peroxide in the range 0.01–2 mM with a detection limit of 8 µM. The linear relative coefficient r = 0.998. The sensor showed rapid response, good reproducibility (relative standard deviation = 2.4%) and a long lifetime of at least two months. The proposed sensor was used to determine a 30% H2O2 solution that has been stored for a long time. The results were in good agreement with the standard volumetric method.\",\"PeriodicalId\":7814,\"journal\":{\"name\":\"Analytical Communications\",\"volume\":\"9 1\",\"pages\":\"195-197\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1039/A901946D\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/A901946D","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Sol–gel horseradish peroxidase biosensor for the chemiluminescent flow determination of hydrogen peroxide
A chemiluminescent (CL) H2O2 sensor based on horseradish peroxidase (HRP) immobilized by the sol–gel method has been proposed in this paper. A new process of fabricating a non-cracking HRP encapsulated sol–gel membrane has been suggested. Flow injection analysis (FIA) was used to give reproducible results. Under optimum conditions, the sensor showed a linear response toward hydrogen peroxide in the range 0.01–2 mM with a detection limit of 8 µM. The linear relative coefficient r = 0.998. The sensor showed rapid response, good reproducibility (relative standard deviation = 2.4%) and a long lifetime of at least two months. The proposed sensor was used to determine a 30% H2O2 solution that has been stored for a long time. The results were in good agreement with the standard volumetric method.