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引用次数: 13
摘要
本研究在不同盐度和ph的F/2培养基中培养海藻类水四瓣藻(Tetraselmis suecica)，研究应激对藻类细胞生产生物燃料的影响。用尼罗红染料测定了在盐度胁迫(1 M NaCl)和pH (pH 7 ~ 9)条件下水藻细胞组装的中性脂质(甘油三酯)。在1 Molars时，盐度对水藻细胞中的甘油三酯有刺激作用，而pH的影响是可变的。采用气相色谱-质谱联用技术对经盐酸在甲醇中直接反式酯化后的棘球藻细胞进行脂肪酸谱分析。结果表明，高盐度是生产生物柴油的理想条件，因为高盐度增加了水四螺生产的单不饱和脂肪酸。
Effect of Salinity and pH on Fatty Acid Profile of The Green Algae Tetraselmis suecica
In this study, the marine alga Tetraselmis suecica was cultured in F/2 medium with various rang of salinity and pH. The influence of stressed algal cells to produce biofuels was studied. The neutral lipid (triacylglycerol) assembled by T. suecica was determined using Nile Red dye under salinity stress (up to 1 M NaCl) and pH (pH 7 to 9). Salinity showed a stimulating effect on triacylglycerol in the algal cells at the 1 Molars, while the effect of pH was changeable. Then fatty acid profile of T. suecica cells was evaluated by gas chromatography–mass spectrometry (GC-MS) after direct trans-esterification with hydrochloric acid in methanol. It was shown that, high salinity was ideal for biodiesel production due to increasing of monounsaturated fatty acids produced by Tetraselmis suecica.
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