微胶囊化前后含r -藻红蛋白水提物的抗氧化和抗酪氨酸酶活性

Q3 Environmental Science
T. S. Khatulistiani, D. Oktavia, I. Munifah, E. Marraskuranto
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引用次数: 0

摘要

杜鹃花水提物(HDWE)含有一种深粉红色的红柱绿菊酯(R-PE)色素,其稳定性受温度和pH的影响,并具有生物活性。微胶囊化可能是保存R-PE性质的解决方案。本研究对HDWE乳液进行了表征,并对酪蛋白酸钠和麦芽糖糊精(M-HDWE)微胶囊化前后的RPE含量、抗氧化剂和抗酪氨酸酶活性进行了分析。在4°C蒸馏水中(比例为1:2,w/v)提取24小时。酪蛋白酸钠与麦芽糖糊精包衣比例为1:2;1:4;1:6和1:12。分别采用Brookfield DV2T、ColorFlex EZ Hunterlab、UV-VIS分光光度法和JeolSEM测定乳液(粘度、颜色和R-PE含量)和粉末特性(表面形貌)。采用铁还原抗氧化能力(FRAP)和蘑菇酪氨酸酶测定法测定其抗氧化和抗酪氨酸酶活性。国际委员会Éclairage (CIE) L* a* b*比色法测定结果表明,麦芽糊精越多,粘度越低,乳化液颜色越浓。尽管HDWE的R-PE含量大约高出6倍,但所有M-HDWE处理样品的R-PE含量相同(p 0.05)。M-HDWE 1:2;1:4;1:6;1:12微胶囊比MHDWE 0微胶囊有更多的皱球和更少的裂缝。M-HDWE 1:2的抗氧化活性比HDWE低33.10%,与其他M-HDWE组合物相比最高(17.15±1.27µM/µg提取物,p 0.05)。同时,所有M-HDWE抗酪氨酸酶活性均较低。由此可见,微胶囊化可以保留HDWE的抗氧化活性,但不能保留其抗酪氨酸酶活性
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant and Anti-tyrosinase Activities of Halymenia durvillei Water Extract Containing R-Phycoerythrin Before and After Microencapsulation
Halymenia durvillei water extract (HDWE) contains a dark-pink colored pigment of Rphycoerythrin (R-PE), which its stability is influenced by temperature and pH andpossesses biological activities. Microencapsulation could be the solution to preservingthe nature of R-PE. This study characterized the HDWE emulsion and analyzed the RPE content, antioxidants, and anti-tyrosinase activities before and after microencapsulation using sodium caseinate and maltodextrin (M-HDWE). Halymenia durvillei was extracted in distilled water (ratio 1:2, w/v) for 24 hours at 4 °C. Sodium caseinate to maltodextrin coating ratios were 1:2; 1:4; 1:6 and 1:12. Emulsion (viscosity, color, and R-PE content) and powder characteristics (surface morphology) were determined using Brookfield DV2T, ColorFlex EZ Hunterlab, UV-VIS spectrophotometry, and JeolSEM, respectively. The antioxidant and anti-tyrosinase activities were determined using the Ferric Reducing Antioxidant Power (FRAP) and mushroom tyrosinase assays. The Commision Internationale d’Éclairage (CIE) L* a* b* colorimetry result showed that the more maltodextrin and the lower viscosity, the more intense the emulsion color. Despite HDWE having roughly six-fold more R-PE content, all M-HDWE treatment samples exhibited equal R-PE contents (p 0.05). The M-HDWE 1:2; 1:4; 1:6; and 1:12 microcapsules had more wrinkled spheres and fewer cracks than MHDWE 0. The M-HDWE 1:2 had 33.10% lower antioxidant activity than the HDWE and was the highest compared to other M-HDWE compositions (17.15±1.27 µM/µg extract, p 0.05). Meanwhile, all M-HDWE had low anti-tyrosinase activity. It can be concluded that microencapsulation could be a solution for preserving HDWE’s antioxidant activity but not its anti-tyrosinase activity
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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