研究核糖体RNA折叠辅助蛋白的蛋白质表达管道

Philipp Vierig, R. Börner
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引用次数: 0

摘要

本工作的主要重点是用最小的必要方法建立T7 RNA聚合酶(T7- rnap)和Pumilio和fem3 mRNA结合因子6 (Puf6)的蛋白质合成途径。该蛋白在原核生物中具有高纯度和高活性的表达,并可通过镍- nta亲和层析用六组氨酸标签有效地纯化。这些蛋白质在核糖体RNA (rRNA)的合成和折叠中得到应用。本文介绍了T7和Puf6合成的优化,以确保最高的产量和纯度,这是研究和生物技术应用不可或缺的因素。蛋白质的研究与生物技术应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protein Expression Pipeline for Research on Folding Helper Proteins for Ribosomal RNA
The main focus of this work is to establish a protein synthesis pathway for T7 RNA polymerase (T7-RNAP) and Pumilio and fem3 mRNA binding factors 6 (Puf6) using the minimum necessary methods.The proteins can be cost-effectively expressed pure and highly active in prokaryotes and then effectively purified with a hexahistidine tag via nickel-NTA affinity chromatography. The proteins find application in the synthesis and folding of, for example, ribosomal RNA (rRNA). This paper describes the optimisation of T7 and Puf6 synthesis to ensure the highest possible yield and purity, both indispensable factors for research and biotechnological applications. research and biotechnological application of the proteins.
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