D. Arnaud-Cormos, M. Soueid, R. O’Connor, Y. Percherancier, I. Lagroye, B. Veyret, P. Lévêque
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Delivery system setup and characterization for biological cells exposed to nanosecond pulsed electric field
In this paper, we propose, setup and characterize a delivery system to expose biological cells to nanosecond pulsed electric fields (nsPEF). The delivery system, based on the commercially available xCELLigence device, was characterized through numerical simulations and experimental time-domain measurements. The ability of the delivery system to deliver pulses of 10 ns duration and amplitude higher than 10 MV/m was successfully assessed. The delivery system offers the possibility to conduct multiple experiments with various parameters and simultaneous microscopic observation. Applications of biological cells exposed to nsPEF with the delivery system include real-time impedance measurements and nanoporation related bioexperiments.
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