营养物有效性和有机质质量决定了湖泊生物膜中的细菌群落结构

IF 1.6 4区 环境科学与生态学 Q3 ECOLOGY
RC Seballos, Kevin H. Wyatt, RJ Bernot, S. Brown, S. Chandra, A. Rober
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引用次数: 2

摘要

异养细菌在生态系统过程中发挥着关键作用,但对水生生物膜中细菌群落结构的形成因素知之甚少,特别是在湖泊中。利用分子16S rRNA技术评价了资源控制对亚高山寡营养湖泊生物膜细菌群落结构的影响。我们控制营养物质(氮和磷;在一个全因子设计中,在光照和黑暗条件下(即分别有和没有自养),无机(岩石)和有机(木材)基质上(NP)和葡萄糖(G),使用营养扩散基质原位20 d。在有营养(NP, NP+G)和没有营养(对照,NP+G)处理之间,群落结构的分离模式明显不同。G-only)表明,无论基质类型或光照有效性如何,营养物对群落结构的影响比有机质更强烈。在有机和无机基质上,单营养物处理(NP)和单营养物处理(NP+G)在群落结构上的进一步分离表明,一旦营养物限制得到缓解,有机质质量对群落结构的形成起着重要作用。不同处理间6门3纲19属的相对丰度差异揭示了(1)不同分类群的资源需求差异,(2)种间相互作用对组成的影响,以及(3)个别分类群参与难降解有机质分解的潜力。我们的研究结果揭示了营养物质和有机物质量在塑造细菌群落结构中的作用,这是弥合水生环境中微生物组成和生态系统功能之间知识差距的关键一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nutrient availability and organic matter quality shape bacterial community structure in a lake biofilm
Heterotrophic bacteria play a key role in ecosystem processes, but little is known about the factors that shape bacterial community structure in aquatic biofilms, especially in lakes. We used molecular techniques (16S rRNA) to evaluate resource controls on biofilm bacterial community structure in an oligotrophic subalpine lake. We manipulated nutrients (nitrogen and phosphorus; NP) and glucose (G) on inorganic (rock) and organic (wood) substrates under light and dark conditions (i.e. with and without autotrophy, respectively) in a full factorial design using nutrient diffusing substrates in situ for 20 d. Distinct patterns of separation in community structure between treatments with nutrients (NP, NP+G) and without nutrients (control, G-only) indicated that community structure was more strongly influenced by nutrients than organic matter irrespective of substrate type or light availability. Further separation in community structure between treatments with nutrients only (NP) and nutrients with glucose (NP+G) on both organic and inorganic substrates indicated that once nutrient limitation was alleviated, organic matter quality played an important role in shaping community structure. Differences in the relative abundance of 6 phyla, 3 classes, and 19 genera among treatments revealed (1) contrasting taxa-specific resource requirements, (2) the influence of interspecific interactions on composition, and (3) the potential for individual taxa to participate in the decomposition of recalcitrant organic matter. Our findings provide insight into the role that nutrients and organic matter quality play in shaping bacterial community structure, which is a critical step in bridging the knowledge gap between microbial composition and ecosystem function within aquatic environments.
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来源期刊
Aquatic Microbial Ecology
Aquatic Microbial Ecology 环境科学-海洋与淡水生物学
CiteScore
3.30
自引率
0.00%
发文量
8
审稿时长
3.0 months
期刊介绍: AME is international and interdisciplinary. It presents rigorously refereed and carefully selected Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see AME 27:209), Opinion Pieces (previously called ''As I See It'') and AME Specials. For details consult the Guidelines for Authors. Papers may be concerned with: Tolerances and responses of microorganisms to variations in abiotic and biotic components of their environment; microbial life under extreme environmental conditions (climate, temperature, pressure, osmolarity, redox, etc.). Role of aquatic microorganisms in the production, transformation and decomposition of organic matter; flow patterns of energy and matter as these pass through microorganisms; population dynamics; trophic interrelationships; modelling, both theoretical and via computer simulation, of individual microorganisms and microbial populations; biodiversity. Absorption and transformation of inorganic material; synthesis and transformation of organic material (autotrophic and heterotrophic); non-genetic and genetic adaptation; behaviour; molecular microbial ecology; symbioses.
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