IN VITRO AND IN VIVO ANTIOXIDANT ACTIVITY OF ETHANOLIC EXTRACT OF MALINKARA ZAPOTA BARK

Many plants possess antioxidant ingredients that provided efficacy by additive or synergistic activities. Manilkara zapota bark (MZB) has been used for the treatment of fever and diarrhea. Antioxidant activity of the ethanol crude extract of bark of Manilkara zapota was assessed in vitro using DPPH, reducing power capacity, total antioxidant, total phenol and total flavonoid content assays at different concentration. The extract exhibited potent antioxidant activity compared to known antioxidant. The potent extract of MZB was tested for in vivo efficacy by carbon tetrachloride (CCl 4 ) induced liver damage rats in hepatoprotective model. CCl 4 produced significant alteration of serum marker enzymes, total bilirubin, total protein and liver weight. The extract significantly restored of these values towards normal compared to control group. Due to its natural origin and potent free radical scavenging ability MZB could be used as a potential preventive intervention for free radical mediated diseases.