毒死蜱中毒对达尼奥鱼体内抗氧化系统的影响

V. V. Dovhaniuk, V. Rosalovsky, Y. Salyha
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引用次数: 0

摘要

本文介绍了浓度为0.5 mg/L毒死蜱对达尼奥雷里奥鱼急性中毒的研究结果;0.75 mg/L和1 mg/L的水族水处理24 h,研究其对tbk活性产物含量、过氧化氢酶活性(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPO)、谷胱甘肽还原酶(GR)含量的影响。在加入浓度为0.75 mg/L和1 mg/L的毒死蜱的水中停留24小时后,我们发现与对照组相比,研究组织中tbk活性产物的含量有所增加。0.5 mg/L毒死蜱对头部和躯干组织中CAT酶活性的线性影响;0.75 mg/L和1mg /L。是显示。以斑马鱼过氧化氢酶活性的增长为背景,毒死蜱作用后24 h内,毒死蜱剂量与鱼体两个研究部位的GPOs活性呈反比关系。在头部和躯干组织中,已经确定了毒死蜱对SOD活性的抑制作用。毒死蜱浓度为0.75 mg/L时,该酶活性最低。所得结果补充了毒死蜱对鱼栖动物的毒性作用以及毒死蜱诱导的达尼奥鱼氧化应激过程的数据。所获得的数据可用于开发毒死蜱和其他磷类有机化合物中毒的其他生化标记物,并用于毒理学和环境实验,同时考虑到在农业生产和日常生活中越来越多地使用杀虫制剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
State of the antioxidant system in Danio rerio fish due to the chlorpyrifos intoxication
The article presents the results of studies of acute intoxication in Danio Rerio fish by chlorpyrifos at concentrations 0.5 mg/L; 0.75 mg/L and 1 mg/L of aquarium water for 24 h and its influence on the content of TBK-active products, catalase activity (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPO), glutathione reductase (GR) in their tissues. After 24 h in the fish staying in water with chloropyrifos added in concentrations 0.75 mg/L and 1 mg/L we have revealed an increase of the content of TBK-active products in the studied tissues compared to the control values. The linear nature of the growth of the CAT enzymatic activity in the tissues of head and torso due to the effects of chloropyrifos in doses of 0.5 mg/L; 0.75 mg/L and 1 mg/L. was shown. On the background of the catalase activity growth in Danio rerio, in 24 h after the action of the toxicant, there was an inversely proportional dependence between the dose of chlorpyrifos and the activity of GPOs in both investigated parts of the fish body. In the tissues of the head and torso, inhibition of SOD activity for the action of chlorpyrifos has been established. The activity of this enzyme was the lowest at the chlorpyrifos concentration 0.75 mg/L. The obtained results complement the data on the toxic effect of chlorpyrifos on ichthyofauna and on the course of chlorpyrifos-induced oxidative stress in Danio rerio fish. The obtained data can be used in the development of additional biochemical markers of chlorpyrifos and other phosphor organic compounds intoxication and in toxicological and environmental experiments, taking into account the growing intensity of the use of insecticidal preparations in agricultural production and everyday life.
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