异多尿原影响葡萄球菌感染小鼠吞噬细胞功能,提高小鼠存活率

E. A. Solenova, S. Pavlova
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摘要

本文介绍了异尿酸原素在葡萄球菌感染模型中对动物存活的影响以及人、动物吞噬细胞功能的研究结果。本研究的目的是研究初给异异尿原素对葡萄球菌感染背景下动物存活的影响,以及对小鼠和人类吞噬细胞功能的影响。材料和方法。采用Kaplan-Meier曲线构建金黄色葡萄球菌J49 ATCC 25923感染Balb/C小鼠模型,评价Balb/C小鼠的存活率。采用流式细胞术观察蛋白胨诱导的吞噬细胞向Balb/C小鼠腹腔的迁移、吞噬细胞(中性粒细胞和单核细胞)对人血液的吸收活性以及它们产生的活性氧中间体(ROIs),研究其对吞噬细胞功能的影响。初步腹腔三次注射异尿素(30 mg/kg)可提高金黄色葡萄球菌J49 ATCC 25923引起的Balb/C小鼠的存活率。同时,异尿酸原素剂量依赖性地激活人中性粒细胞和单核细胞产生活性氧中间体,但对单核细胞和中性粒细胞对异硫氰酸荧光标记的金黄色葡萄球菌j49 ATCC 25923的吞噬活性以及吞噬细胞向小鼠腹腔的迁移没有统计学意义上的显著抑制。因此,初步给予异尿酸原可提高葡萄球菌感染小鼠的存活率,并增加吞噬细胞产生活性氧中间体。所得数据可为进一步研究异尿酸原素的抗菌和免疫作用奠定基础,以寻找治疗葡萄球菌感染的新药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ISOLYQUIRITIGENIN AFFECTS PHAGOCYTES FUNCTIONS AND INCREASES MICE SURVIVAL RATE IN STAPHYLOCOCCAL INFECTION
The results of studying the effect of isoliquiritigenin on animal survival in the model of staphylococcal infection and the function of human and animal phagocytes are presented in this article.The aim of the investigation was to study the effect of an isoliquiritigenin preliminary administration on the survival of animals against the background of staphylococcal infection, as well as on the function of phagocytes in mice and humans.Materials and methods. To assess the survival of Balb/C mice, a model of infection caused by Staphylococcus aureus J49 ATCC 25923 with the construction of Kaplan-Meier curves, was used. The effect on the phagocytes functions was studied by assessing the peptone-induced migration of phagocytes into the abdominal cavity of Balb/C mice, the absorption activity of phagocytes (neutrophils and monocytes) of human blood, as well as their production of reactive oxygen intermediates (ROIs) using а flow cytometry.Results. It was found out that a preliminary triple intraperitoneal administration of isoliquiritigenin (30 mg/kg) increases the survival rate of Balb/C mice in staphylococcal infection caused by Staphylococcus aureus J49 ATCC 25923. At the same time, isoliquiritigenin dose-dependently activates the production of reactive oxygen intermediates by human neutrophils and monocytes without statistically significantly suppressing a phagocytic activity of monocytes and neutrophils against fluoresceinisothiocyanate-labeled S. aureus J 49 ATCC 25923, as well as peptone-induced migration of phagocytes into the abdominal cavity of mice.Conclusion. Thus, a preliminary administration of isoliquiritigenin increases the survival rate of mice with staphylococcal infection and increases the production of reactive oxygen intermediates by phagocytes. The data obtained, can become the basis for further research of antibacterial and immunotropic effects of isoliquiritigenin in order to find new drugs for the treatment of staphylococcal infection.
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