{"title":"干扰素调节因子1和2基因在人肝癌细胞系中的表达分析","authors":"Kazuhiko Nakao , Keisuke Nakata , Manabu Daikoku , Kaoru Inokuchi , Tasuku Nomura , Akio Ido , Shotaro Tsuruta , Yuji Kato , Nobuko Ishii , Shigenobu Nagataki","doi":"10.1016/0928-4346(96)00268-X","DOIUrl":null,"url":null,"abstract":"<div><p>The transcription activator, Interferon regulatory factor-1 (IRF-1), has been shown to function as a tumor suppressor and the structurally related interferon regulatory factor-2 (IRF-2) as a counterpart. The loss of IRF-1 function or overexpression of IRF-2 are possible mechanisms of cancer development. In the present study, IRF-1 and IRF-2 gene expression was analyzed in human hepatoma cell lines (HuH7, huH 1, HepG2 and PLC/PRF/5) and the normal liver. We found a small amount of exon-skipped IRF-1 mRNA in HuH7 and huH 1, and the low level of IRF-1 mRNA in PLC/PRF/5, but we could not find the overexpression of IRF-2 mRNA in any hepatoma cells lines. Thus, it is possible that hepatocarcinogenesis involves some alterations in expression of IRF-1 gene.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":"4 6","pages":"Pages 351-357"},"PeriodicalIF":0.0000,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00268-X","citationCount":"0","resultStr":"{\"title\":\"Analysis of interferon regulatory factor 1 and 2 gene expression in human hepatoma cell lines\",\"authors\":\"Kazuhiko Nakao , Keisuke Nakata , Manabu Daikoku , Kaoru Inokuchi , Tasuku Nomura , Akio Ido , Shotaro Tsuruta , Yuji Kato , Nobuko Ishii , Shigenobu Nagataki\",\"doi\":\"10.1016/0928-4346(96)00268-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The transcription activator, Interferon regulatory factor-1 (IRF-1), has been shown to function as a tumor suppressor and the structurally related interferon regulatory factor-2 (IRF-2) as a counterpart. The loss of IRF-1 function or overexpression of IRF-2 are possible mechanisms of cancer development. In the present study, IRF-1 and IRF-2 gene expression was analyzed in human hepatoma cell lines (HuH7, huH 1, HepG2 and PLC/PRF/5) and the normal liver. We found a small amount of exon-skipped IRF-1 mRNA in HuH7 and huH 1, and the low level of IRF-1 mRNA in PLC/PRF/5, but we could not find the overexpression of IRF-2 mRNA in any hepatoma cells lines. Thus, it is possible that hepatocarcinogenesis involves some alterations in expression of IRF-1 gene.</p></div>\",\"PeriodicalId\":13746,\"journal\":{\"name\":\"International Hepatology Communications\",\"volume\":\"4 6\",\"pages\":\"Pages 351-357\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0928-4346(96)00268-X\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Hepatology Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/092843469600268X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Hepatology Communications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092843469600268X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Analysis of interferon regulatory factor 1 and 2 gene expression in human hepatoma cell lines
The transcription activator, Interferon regulatory factor-1 (IRF-1), has been shown to function as a tumor suppressor and the structurally related interferon regulatory factor-2 (IRF-2) as a counterpart. The loss of IRF-1 function or overexpression of IRF-2 are possible mechanisms of cancer development. In the present study, IRF-1 and IRF-2 gene expression was analyzed in human hepatoma cell lines (HuH7, huH 1, HepG2 and PLC/PRF/5) and the normal liver. We found a small amount of exon-skipped IRF-1 mRNA in HuH7 and huH 1, and the low level of IRF-1 mRNA in PLC/PRF/5, but we could not find the overexpression of IRF-2 mRNA in any hepatoma cells lines. Thus, it is possible that hepatocarcinogenesis involves some alterations in expression of IRF-1 gene.
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