激光和动态四极射频治疗对脱垂女性阴道上皮胶原生成蛋白表达和组织弹性的影响

Q3 Medicine
Y. Dobrokhotova, E. Kareva, I. I. Grishin, Natalia A. Kochina, E. V. Krasnoshchok, V. I. Komagorov
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引用次数: 0

摘要

背景。生殖器脱垂严重影响患者的生活质量。各种资料显示,手术治疗后复发率为30 ~ 60%。的目标。测定和比较绝经后患者术后暴露于激光和射频前后胶原代谢蛋白的表达水平,并比较超声弹性成像数据,以证明使用这些暴露方法作为康复方案是合理的。材料和方法。对54例绝经后POP-Q 3级外阴脱垂患者采集组织学标本,术前及康复治疗后行超声检查。采用实时聚合酶链反应法检测I型胶原蛋白、基质金属蛋白酶(MMP-2、MMP-9)和组织金属蛋白酶抑制剂(TIMP-1、TIMP-2)的表达水平。超声检查采用压缩弹性成像技术,分析组织的色阶和变形系数。结果。数据显示射频治疗组与治疗前相比,TIMP-1表达增加了7倍,TIMP-2表达增加了4倍,激光治疗组女性阴道组织中TIMP-2表达比治疗前减少了64倍。射频暴露后阴道组织中TIMP-1和TIMP-2基因的表达显著增加,而激光暴露后TIMP-2基因的表达显著降低,这表明生物标志物对能量治疗的补偿作用,以及这些方法的重塑作用。超声显示,在激光和射频照射后,患者的组织弹性增加。结论。结果支持在术后使用射频和激光治疗作为康复方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of laser and dynamic quadripolar radiofrequency therapies on the expression of collagenogenesis proteins in the vaginal epithelium and tissue elasticity in women with genital prolapse
Background. Genital prolapse dramatically affects the quality of life of patients. Various sources show that the relapse rate after surgical treatment is 30 to 60%. Aim. To determine and compare the expression levels of collagen metabolism proteins before and after exposure to laser and radio frequency in the postoperative period in postmenopausal patients and to compare ultrasound elastography data to justify using these exposure methods as a rehabilitation program. Materials and methods. Histological specimens were collected from 54 patients with postmenopausal POP-Q grade 3 genital prolapse, and an ultrasound examination was made before surgery and after rehabilitation therapy. A real-time polymerase chain reaction method was used to assess the expression level of type I collagen, matrix metalloproteinases (MMP-2, MMP-9), and tissue inhibitors of metalloproteinases (TIMP-1, TIMP-2). Ultrasound examination was performed using the technology of compression elastography with the analysis of the color scale and the coefficient of tissue deformation. Results. Data were obtained on the increase in the expression of TIMP-1 by 7 times and TIMP-2 by 4 times in the radiofrequency therapy group compared to the group before treatment, as well as a 64-fold decrease in TIMP-2 expression in the vaginal tissue of women in the group after laser therapy compared to the group before treatment. A significant increase in the expression of TIMP-1 and TIMP-2 genes in vaginal tissue after radiofrequency exposure and a decrease in TIMP-2 after laser exposure demonstrate the compensatory effect of biological markers in response to energy therapy, as well as the remodeling effect of these methods. According to ultrasound, tissue elasticity in patients increased after laser and radiofrequency exposure. Conclusion. The results support using radiofrequency and laser therapy in the postoperative period as rehabilitation methods.
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来源期刊
Gynecology
Gynecology Medicine-Obstetrics and Gynecology
CiteScore
0.70
自引率
0.00%
发文量
52
审稿时长
8 weeks
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