乙磺酰亚胺对谷氨酸诱导的C6神经胶质细胞毒性和氧化应激的保护作用

B. Sahin
{"title":"乙磺酰亚胺对谷氨酸诱导的C6神经胶质细胞毒性和氧化应激的保护作用","authors":"B. Sahin","doi":"10.7176/jstr/7-08-10","DOIUrl":null,"url":null,"abstract":"Purpose : The current study was intended to investigate the potential effect of ethosuximide on glutamate-induced cytotoxicity and oxidative stress in C6 glial cells. Material and Methods: In our study, the C6 cell line was used. To see how ethosuximide affected glutamate-induced cytotoxicity, four cell groups were created. The control group was without any treatment. Cells in the glutamate group were exposed to 10 mM glutamate for 24 h. Cells in the ethosuximide group were treated with various concentrations (5, 10, 20, and 40 μM) of ethosuximide for 24 h. Cells in the ethosuximide + glutamate group were pre-treated with various concentrations of ethosuximide for 1 h and then treated with glutamate for 24 h. The cell viability was measured using an XTT assay.Total oxidant status (TOS) and Total antioxidant status (TAS) in the cells were measured by commercial kits. Results: Ethosuximide at the concentrations of 10, 20, and 40 μM significantly increased the cell viability in C6 cells (p < 0.001). Ethosuximide at the concentration of 40 μM significantly decreased the TOS levels (p < 0.05) whereas didn’t alter the TAS levels (p > 0.05). Conclusion: Ethosuximide can cause neuroprotective effect on C6 cells. Additionally, one of the potential mechanisms involved in this effect is inhibition of oxidative stress. Alzheimer’s, Parkinson’s, Huntington’s","PeriodicalId":14256,"journal":{"name":"International Journal of Scientific and Technological Research","volume":"13 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Protective Effect of Ethosuximide against Glutamate-Induced Cytotoxicity and Oxidative Stress in C6 Glial Cells\",\"authors\":\"B. Sahin\",\"doi\":\"10.7176/jstr/7-08-10\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Purpose : The current study was intended to investigate the potential effect of ethosuximide on glutamate-induced cytotoxicity and oxidative stress in C6 glial cells. Material and Methods: In our study, the C6 cell line was used. To see how ethosuximide affected glutamate-induced cytotoxicity, four cell groups were created. The control group was without any treatment. Cells in the glutamate group were exposed to 10 mM glutamate for 24 h. Cells in the ethosuximide group were treated with various concentrations (5, 10, 20, and 40 μM) of ethosuximide for 24 h. Cells in the ethosuximide + glutamate group were pre-treated with various concentrations of ethosuximide for 1 h and then treated with glutamate for 24 h. The cell viability was measured using an XTT assay.Total oxidant status (TOS) and Total antioxidant status (TAS) in the cells were measured by commercial kits. Results: Ethosuximide at the concentrations of 10, 20, and 40 μM significantly increased the cell viability in C6 cells (p < 0.001). Ethosuximide at the concentration of 40 μM significantly decreased the TOS levels (p < 0.05) whereas didn’t alter the TAS levels (p > 0.05). Conclusion: Ethosuximide can cause neuroprotective effect on C6 cells. Additionally, one of the potential mechanisms involved in this effect is inhibition of oxidative stress. Alzheimer’s, Parkinson’s, Huntington’s\",\"PeriodicalId\":14256,\"journal\":{\"name\":\"International Journal of Scientific and Technological Research\",\"volume\":\"13 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Scientific and Technological Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7176/jstr/7-08-10\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Scientific and Technological Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7176/jstr/7-08-10","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

目的:本研究旨在探讨乙氧亚胺对谷氨酸诱导的C6神经胶质细胞的细胞毒性和氧化应激的潜在影响。材料与方法:本研究采用C6细胞系。为了观察乙磺酰亚胺如何影响谷氨酸诱导的细胞毒性,我们创建了四个细胞组。对照组不做任何治疗。谷氨酸组细胞暴露于10 mM谷氨酸中24 h,乙氧亚胺组细胞用不同浓度(5、10、20和40 μM)的乙氧亚胺处理24 h,乙氧亚胺+谷氨酸组细胞用不同浓度的乙氧亚胺预处理1 h,然后用谷氨酸处理24 h,采用XTT法测定细胞活力。用商业试剂盒测定细胞总氧化状态(TOS)和总抗氧化状态(TAS)。结果:10、20、40 μM浓度的乙氧亚胺显著提高C6细胞的细胞活力(p < 0.001)。40 μM乙氧亚胺可显著降低小鼠TOS水平(p < 0.05),但对TAS水平无显著影响(p < 0.05)。结论:乙磺酰亚胺对C6细胞有一定的神经保护作用。此外,这种作用的潜在机制之一是抑制氧化应激。阿尔茨海默氏症,帕金森症,亨廷顿氏症
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective Effect of Ethosuximide against Glutamate-Induced Cytotoxicity and Oxidative Stress in C6 Glial Cells
Purpose : The current study was intended to investigate the potential effect of ethosuximide on glutamate-induced cytotoxicity and oxidative stress in C6 glial cells. Material and Methods: In our study, the C6 cell line was used. To see how ethosuximide affected glutamate-induced cytotoxicity, four cell groups were created. The control group was without any treatment. Cells in the glutamate group were exposed to 10 mM glutamate for 24 h. Cells in the ethosuximide group were treated with various concentrations (5, 10, 20, and 40 μM) of ethosuximide for 24 h. Cells in the ethosuximide + glutamate group were pre-treated with various concentrations of ethosuximide for 1 h and then treated with glutamate for 24 h. The cell viability was measured using an XTT assay.Total oxidant status (TOS) and Total antioxidant status (TAS) in the cells were measured by commercial kits. Results: Ethosuximide at the concentrations of 10, 20, and 40 μM significantly increased the cell viability in C6 cells (p < 0.001). Ethosuximide at the concentration of 40 μM significantly decreased the TOS levels (p < 0.05) whereas didn’t alter the TAS levels (p > 0.05). Conclusion: Ethosuximide can cause neuroprotective effect on C6 cells. Additionally, one of the potential mechanisms involved in this effect is inhibition of oxidative stress. Alzheimer’s, Parkinson’s, Huntington’s
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信