{"title":"微波辅助样品提取-高效液相色谱(HPLC)法定量分析水生生物样品中水解氨基酸的验证","authors":"G. Jayasinghe, B. Jinadasa","doi":"10.4038/SLJAS.V24I1.7564","DOIUrl":null,"url":null,"abstract":"A sensitive and widely applicable High-Performance Liquid Chromatography (HPLC) with fluorescence detector (FLD) method for determination hydrolysed amino acids in fish and aquatic products was developed and validated through study. The method involved a microwave-assisted sample extraction and protein hydrolysed with the hydrochloric acid and propionic acid. The separation was performed by Shim-pack HR-ODS column with a gradient mobile phase. The total separation time was 25 min per run. The method showed satisfactory linearity, repeatability, accuracy, and limit of detection for 21 amino acids with the 1-100 mg/L calibration range based on 10 µL injection. The fish, fish feed, canned fish and seaweeds samples were analysed successfully by using this developed method.","PeriodicalId":21784,"journal":{"name":"Sri Lanka Journal of Aquatic Sciences","volume":"51 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Validation of microwave-assisted sample extraction-high performance liquid chromatography (HPLC) method for quantitative analysis of hydrolysed amino acids in aquatic biological samples\",\"authors\":\"G. Jayasinghe, B. Jinadasa\",\"doi\":\"10.4038/SLJAS.V24I1.7564\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A sensitive and widely applicable High-Performance Liquid Chromatography (HPLC) with fluorescence detector (FLD) method for determination hydrolysed amino acids in fish and aquatic products was developed and validated through study. The method involved a microwave-assisted sample extraction and protein hydrolysed with the hydrochloric acid and propionic acid. The separation was performed by Shim-pack HR-ODS column with a gradient mobile phase. The total separation time was 25 min per run. The method showed satisfactory linearity, repeatability, accuracy, and limit of detection for 21 amino acids with the 1-100 mg/L calibration range based on 10 µL injection. The fish, fish feed, canned fish and seaweeds samples were analysed successfully by using this developed method.\",\"PeriodicalId\":21784,\"journal\":{\"name\":\"Sri Lanka Journal of Aquatic Sciences\",\"volume\":\"51 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-03-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sri Lanka Journal of Aquatic Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4038/SLJAS.V24I1.7564\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sri Lanka Journal of Aquatic Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4038/SLJAS.V24I1.7564","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Validation of microwave-assisted sample extraction-high performance liquid chromatography (HPLC) method for quantitative analysis of hydrolysed amino acids in aquatic biological samples
A sensitive and widely applicable High-Performance Liquid Chromatography (HPLC) with fluorescence detector (FLD) method for determination hydrolysed amino acids in fish and aquatic products was developed and validated through study. The method involved a microwave-assisted sample extraction and protein hydrolysed with the hydrochloric acid and propionic acid. The separation was performed by Shim-pack HR-ODS column with a gradient mobile phase. The total separation time was 25 min per run. The method showed satisfactory linearity, repeatability, accuracy, and limit of detection for 21 amino acids with the 1-100 mg/L calibration range based on 10 µL injection. The fish, fish feed, canned fish and seaweeds samples were analysed successfully by using this developed method.
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