Shukhrat Gaibullaev, Feruza Berdymuradov, K. Zakhidov, D. Aronbaev, Sergey D. Aronbaev
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DEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF TINIDAZOLE IN PHARMACEUTICAL PRODUCTS
A simple and economical UV spectrophotometric method for the quantitative assessment of tinidazole has been developed and tested. Selected wavelength of maximum absorption λ max . for a solution of tinidazole in phosphate buffer (pH = 6.86) is 317.3 nm. The calibration graph shows linearity at the selected wavelength and obeys the Lambert-Bouguer-Beer law in the concentration range of 3.0-30 μg / ml with a correlation coefficient of at least 0.992. Studies have been conducted on the extraction of tinidazole in pharmaceuticals, which is 97.9 - 100.4%, which confirms the accuracy of the proposed method. The metrological evaluation of the developed spectrophotometric method showed good reproducibility and accuracy of the determination of tinidazole in mono- and multicomponent pharmaceutical products from various manu-facturers. The relative error in the determination of tinidazole does not exceed 2%. Statistical processing of the data obtained shows that the proposed method can be successfully applied for routine analysis of the stability of tinidazole in laboratories for quality control of drugs.
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