卵磷脂对体外亚砷酸钠作用下非洲绿猴肾细胞膜损伤的干预作用

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-07-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.04.013

Tingxin Wang, Ya-lou Zhang, Ji-Wen Liu, Shengling Wang

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Na+ ,K+-ATPase activities of membrane were measured by spectrophotometry, and membrane phospholipids composition including phosphatidylserine (PS), phosphatidylethano-lamine (PE), phosphatidylcholine (PC) and sphingmyelin (SM) were measured by high performance liquid chromatography (HPLC). Results The Na~, K+-ATPase activities of membrane of control group, model group, high concentration of lecithin and arsenic group, low concentration of lecithin and arsenic group were (0.962 ± 0.081) × 106, (0.544 ± 0.037) × 106, (0.647 ± 0.043) x 106, (0.550±Compared with control group, the Na+ ,K+-ATPase activities of other 3 groups were significantly reduced (all P 0.05). Compared with control group[(0.087 ± 0.003), (0.127 ± 0.053), (0.588 ± 0.105),(0.071 ± 0.029)g/L], PS, PE, PC, SM levels in model group[(0.051 ± 0.018), (0.073 + 0.030), (0.240 ±0.038), (0.047 ± 0.121 )g/L] were significantly lower(all P 0.05), but SM[(0.057 ± 0.004)g/L] significantly decreased(P 0.05]. 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Results The Na~, K+-ATPase activities of membrane of control group, model group, high concentration of lecithin and arsenic group, low concentration of lecithin and arsenic group were (0.962 ± 0.081) × 106, (0.544 ± 0.037) × 106, (0.647 ± 0.043) x 106, (0.550±Compared with control group, the Na+ ,K+-ATPase activities of other 3 groups were significantly reduced (all P 0.05). Compared with control group[(0.087 ± 0.003), (0.127 ± 0.053), (0.588 ± 0.105),(0.071 ± 0.029)g/L], PS, PE, PC, SM levels in model group[(0.051 ± 0.018), (0.073 + 0.030), (0.240 ±0.038), (0.047 ± 0.121 )g/L] were significantly lower(all P 0.05), but SM[(0.057 ± 0.004)g/L] significantly decreased(P 0.05]. Compared with model group,the levels of PS, PE, PC, SM in high concentration of lecithin and arsenic group were significantly higher(all P 0.05), and PC was significantly higher(P < 0.05). 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引用次数: 0

摘要

目的观察卵磷脂对亚砷酸钠(NaAsO2)作用下非洲绿猴肾细胞(Vero)膜的影响。方法将体外培养的Vero细胞分为4组:对照组(生理盐水)、模型组(2.20 mg/L NaAsO2)、高浓度卵磷脂和砷组(53.33mg/L卵磷脂+ 2.20 mg/L NaAsO2)、低浓度卵磷脂和砷组(13.32 mg/L卵磷脂+ 2.20 mg/L NaAsO2)，每组6瓶，每2 d换一次培养基，培养120 h，分光光度法测定细胞膜Na+、K+- atp酶活性。采用高效液相色谱法测定膜磷脂组成，包括磷脂酰丝氨酸(PS)、磷脂酰乙醇胺(PE)、磷脂酰胆碱(PC)和鞘髓磷脂(SM)。结果对照组、模型组、高浓度卵磷脂和砷组、低浓度卵磷脂和砷组细胞膜Na~、K+- atp酶活性分别为(0.962±0.081)× 106、(0.544±0.037)× 106、(0.647±0.043)× 106、(0.550±)，与对照组比较，其他3组细胞膜Na+、K+- atp酶活性均显著降低(均P 0.05)。与对照组[(0.087±0.003)、(0.127±0.053)、(0.588±0.105)、(0.071±0.029)g/L]相比，模型组PS、PE、PC、SM水平[(0.051±0.018)、(0.073 + 0.030)、(0.240±0.038)、(0.047±0.121)g/L]显著降低(P 0.05)， SM[(0.057±0.004)g/L]显著降低(P 0.05)。与模型组相比，高浓度卵磷脂和砷组PS、PE、PC、SM水平显著升高(均P 0.05)， PC水平显著升高(P < 0.05)。结论高浓度卵磷脂对亚砷酸钠暴露的Vero细胞膜有一定的保护作用。关键词:亚砷酸盐;磷脂酰胆碱;细胞膜;Na + - k +交换腺苷三磷酸酶

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Intervention effect of lecithin on cell membrane injury of African green monkey kidney exposed to sodium arsenite in vitro

Objective To observe the lecithin's effect on membrane of African green monkey kidney cells (Vero) exposed to sodium arsenite(NaAsO2). Methods Vero cells cultured in vitro were divided into 4 groups:control group (saline), model group (2.20 mg/L NaAsO2), high eoncentration of lecithin and arsenic group (53.33mg/L lecithin + 2.20 mg/L NaAsO2), low eoncentration of lecithin and arsenic group( 13.32 mg/L lecithin + 2.20 mg/L NaAsO2), 6 bottles of cells in each group, medium was changed every 2 days, cultured for 120 h. Na+ ,K+-ATPase activities of membrane were measured by spectrophotometry, and membrane phospholipids composition including phosphatidylserine (PS), phosphatidylethano-lamine (PE), phosphatidylcholine (PC) and sphingmyelin (SM) were measured by high performance liquid chromatography (HPLC). Results The Na~, K+-ATPase activities of membrane of control group, model group, high concentration of lecithin and arsenic group, low concentration of lecithin and arsenic group were (0.962 ± 0.081) × 106, (0.544 ± 0.037) × 106, (0.647 ± 0.043) x 106, (0.550±Compared with control group, the Na+ ,K+-ATPase activities of other 3 groups were significantly reduced (all P 0.05). Compared with control group[(0.087 ± 0.003), (0.127 ± 0.053), (0.588 ± 0.105),(0.071 ± 0.029)g/L], PS, PE, PC, SM levels in model group[(0.051 ± 0.018), (0.073 + 0.030), (0.240 ±0.038), (0.047 ± 0.121 )g/L] were significantly lower(all P 0.05), but SM[(0.057 ± 0.004)g/L] significantly decreased(P 0.05]. Compared with model group,the levels of PS, PE, PC, SM in high concentration of lecithin and arsenic group were significantly higher(all P 0.05), and PC was significantly higher(P < 0.05). Conclusions High concentration lecithin has certain protective effect on Vero cell membrane exposured to sodium arsenite. Key words: Arsenites ; Phosphatidylcholines; Cell membrane; Na+-K+-exchanging ATPase

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.