Dalia M. Abdel Hamid , Safa F. Abdel El-Ghani , Mohamed M. Khashaba
{"title":"纳米羟基磷灰石硅胶的表征及其与索氏胶膏联合对新西兰兔骨形成的影响","authors":"Dalia M. Abdel Hamid , Safa F. Abdel El-Ghani , Mohamed M. Khashaba","doi":"10.1016/j.fdj.2018.05.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Characterization of nano-hydroxyapatite (n-HAp) silica gel bone substitute and evaluation of its combined effect with Solcoseryl paste on bone formation experimentally in New Zealand rabbits.</p></div><div><h3>Materials and methods</h3><p>Characterization of the n-HAp silica gel granules (NanoBone<sup>®</sup>, Artoss GmbH, German) was done by the SEM, EDX analysis and TEM. Simulated bone defect (10 × 5 × 1 mm) was constructed from metallic mold to standardize the amount of the grafting materials. Two defects (10 × 5 × 1 mm) were created in the parietal bones of 12 New Zealand White rabbits. The rabbits were randomly divided into 4 groups with 3 rabbits/group (n = 6 defects/group). Group (C): The defects were left empty for natural bone healing (control group), group (S): The defects were filled with the pre-weighed Solcoseryl<sup>®</sup> paste (Legacy Pharmaceuticals Switzerland GmbH, Switzerland), group (N): the defects were filled with the pre-weighed NanoBone and group (SN): the defects were filled with Solcoseryl paste/NanoBone mixture (1:1 by Vol. %). The rabbits were sacrified on day 14; the defects were removed, prepared for histological assessment and histomorphometric analysis. The amount of bone formation was statistically analyzed by ANOVA and Post-hoc test (p < 0.05).</p></div><div><h3>Results</h3><p>The SEM revealed the highly rough, porous n-HAp silica gel granules. The TEM revealed the interconnected porosities within the silica matrix and the platelet like shape of the n-HAp crystals. The Ca: P ratio of the n-HAp silica gel granules was 1.74. Histological evaluation revealed absence of new bone formation in group C while calcification in most of osteoid tissue areas was evident in all experimental groups with evident wide bone marrow cavities in S group. The NS group possessed the highest significant bone area percentage.</p></div><div><h3>Conclusions</h3><p>The interconnected porosities and the highly rough surface of n-HAp silica gel granules provided biomimetic bone substitute material that promotes new bone formation. Solcoseryl/NanoBone mixture (1:1 vol %) has a synergistic osteoinductive effect quantitatively and qualitatively on bone formation.</p></div><div><h3>Clinical significance</h3><p>Acceleration of post-operative bone healing in certain clinical cases where physiologic or pathologic conditions could impair the vascularization process.</p></div>","PeriodicalId":100562,"journal":{"name":"Future Dental Journal","volume":"4 2","pages":"Pages 279-287"},"PeriodicalIF":0.0000,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fdj.2018.05.007","citationCount":"4","resultStr":"{\"title\":\"Characterization of nano-hydroxyapatite silica gel and evaluation of its combined effect with Solcoseryl paste on bone formation: An experimental study in New Zealand rabbits\",\"authors\":\"Dalia M. Abdel Hamid , Safa F. Abdel El-Ghani , Mohamed M. Khashaba\",\"doi\":\"10.1016/j.fdj.2018.05.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Characterization of nano-hydroxyapatite (n-HAp) silica gel bone substitute and evaluation of its combined effect with Solcoseryl paste on bone formation experimentally in New Zealand rabbits.</p></div><div><h3>Materials and methods</h3><p>Characterization of the n-HAp silica gel granules (NanoBone<sup>®</sup>, Artoss GmbH, German) was done by the SEM, EDX analysis and TEM. Simulated bone defect (10 × 5 × 1 mm) was constructed from metallic mold to standardize the amount of the grafting materials. Two defects (10 × 5 × 1 mm) were created in the parietal bones of 12 New Zealand White rabbits. The rabbits were randomly divided into 4 groups with 3 rabbits/group (n = 6 defects/group). Group (C): The defects were left empty for natural bone healing (control group), group (S): The defects were filled with the pre-weighed Solcoseryl<sup>®</sup> paste (Legacy Pharmaceuticals Switzerland GmbH, Switzerland), group (N): the defects were filled with the pre-weighed NanoBone and group (SN): the defects were filled with Solcoseryl paste/NanoBone mixture (1:1 by Vol. %). The rabbits were sacrified on day 14; the defects were removed, prepared for histological assessment and histomorphometric analysis. The amount of bone formation was statistically analyzed by ANOVA and Post-hoc test (p < 0.05).</p></div><div><h3>Results</h3><p>The SEM revealed the highly rough, porous n-HAp silica gel granules. The TEM revealed the interconnected porosities within the silica matrix and the platelet like shape of the n-HAp crystals. The Ca: P ratio of the n-HAp silica gel granules was 1.74. Histological evaluation revealed absence of new bone formation in group C while calcification in most of osteoid tissue areas was evident in all experimental groups with evident wide bone marrow cavities in S group. The NS group possessed the highest significant bone area percentage.</p></div><div><h3>Conclusions</h3><p>The interconnected porosities and the highly rough surface of n-HAp silica gel granules provided biomimetic bone substitute material that promotes new bone formation. Solcoseryl/NanoBone mixture (1:1 vol %) has a synergistic osteoinductive effect quantitatively and qualitatively on bone formation.</p></div><div><h3>Clinical significance</h3><p>Acceleration of post-operative bone healing in certain clinical cases where physiologic or pathologic conditions could impair the vascularization process.</p></div>\",\"PeriodicalId\":100562,\"journal\":{\"name\":\"Future Dental Journal\",\"volume\":\"4 2\",\"pages\":\"Pages 279-287\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.fdj.2018.05.007\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future Dental Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2314718018300272\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future Dental Journal","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2314718018300272","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Characterization of nano-hydroxyapatite silica gel and evaluation of its combined effect with Solcoseryl paste on bone formation: An experimental study in New Zealand rabbits
Objective
Characterization of nano-hydroxyapatite (n-HAp) silica gel bone substitute and evaluation of its combined effect with Solcoseryl paste on bone formation experimentally in New Zealand rabbits.
Materials and methods
Characterization of the n-HAp silica gel granules (NanoBone®, Artoss GmbH, German) was done by the SEM, EDX analysis and TEM. Simulated bone defect (10 × 5 × 1 mm) was constructed from metallic mold to standardize the amount of the grafting materials. Two defects (10 × 5 × 1 mm) were created in the parietal bones of 12 New Zealand White rabbits. The rabbits were randomly divided into 4 groups with 3 rabbits/group (n = 6 defects/group). Group (C): The defects were left empty for natural bone healing (control group), group (S): The defects were filled with the pre-weighed Solcoseryl® paste (Legacy Pharmaceuticals Switzerland GmbH, Switzerland), group (N): the defects were filled with the pre-weighed NanoBone and group (SN): the defects were filled with Solcoseryl paste/NanoBone mixture (1:1 by Vol. %). The rabbits were sacrified on day 14; the defects were removed, prepared for histological assessment and histomorphometric analysis. The amount of bone formation was statistically analyzed by ANOVA and Post-hoc test (p < 0.05).
Results
The SEM revealed the highly rough, porous n-HAp silica gel granules. The TEM revealed the interconnected porosities within the silica matrix and the platelet like shape of the n-HAp crystals. The Ca: P ratio of the n-HAp silica gel granules was 1.74. Histological evaluation revealed absence of new bone formation in group C while calcification in most of osteoid tissue areas was evident in all experimental groups with evident wide bone marrow cavities in S group. The NS group possessed the highest significant bone area percentage.
Conclusions
The interconnected porosities and the highly rough surface of n-HAp silica gel granules provided biomimetic bone substitute material that promotes new bone formation. Solcoseryl/NanoBone mixture (1:1 vol %) has a synergistic osteoinductive effect quantitatively and qualitatively on bone formation.
Clinical significance
Acceleration of post-operative bone healing in certain clinical cases where physiologic or pathologic conditions could impair the vascularization process.
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