光剥夺引起大鼠全身盖褪黑激素MT1受体的表达特征

I. S. Sobolevskaya, M. I. Krasnobaeva, O. D. Myadelets
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摘要

背景。现在已经确定,昼夜节律时钟同步工作的任何变化都会导致人体调节系统的破坏,这包括代谢紊乱和组织损伤的发展。目的:研究光剥夺条件下白雄性大鼠皮肤MT1褪黑素受体表达的动态变化。方法。本研究采用免疫组织化学方法研究了褪黑激素受体(MT1)的表达特征。实验选用体重170 ~ 220 g的远交系雄性大鼠,分为2组;完好无损-动物在标准的固定照明条件下饲养(12小时光照/ 12小时黑暗);第二组:24小时黑暗条件下的模拟光剥夺动物。结果。MT1褪黑激素受体在一般被膜中的表达具有强烈的昼夜依赖性。在表皮和皮脂腺细胞中,第14天MT1表达量显著降低,第21天MT1表达量急剧升高。在表皮角质形成细胞中,在整个研究过程中注意到指标变化的波浪形特征:增加(第7天)-减少(第14天)-急剧增加(第21天)。结论。最初,光剥夺导致免疫阳性细胞数量减少,随后其水平代偿性增加,这是机体对时间破坏适应机制的表现之一。
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Features of the expression of melatonin MT1 receptors in the general cover of rats caused by light deprivation
Background. It has now been established that any change in the synchronized work of the circadian clock leads to a disruption of the body's regulatory systems, which consists in the development of metabolic disorders and tissue damage. Objective: to study the dynamics of the expression of the MT1 melatonin receptor in the skin of white male rats under light deprivation. Methods. In this work, using immunohistochemistry, the features of the expression of receptors for melatonin (MT1) were studied. The experiments were performed on outbred male rats weighing 170-220 g, which were divided into 2 groups; intact - animals kept under standard fixed lighting conditions (12 h light / 12 h dark); Group 2 - animals with simulated light deprivation in conditions of round-the-clock darkness (24 hours of darkness). Results. A strong circadian dependence of the expression of MT1 melatonin receptors in the general integument was established. In the cells of the epidermis and sebaceous glands, by the 14th day, MT1 expression significantly decreased, which then sharply increased by the 21st day. In the keratinocytes of the epidermis, a wavelike character of changes in indicators is noted throughout the study: an increase (7th day) - a decrease (14th day) - a sharp increase (21st day). Conclusion. Initially, light deprivation leads to a decrease in the number of immunopositive cells, and then their level increases compensatory, which is one of the manifestations of the body's adaptation mechanisms to chronodestruction.
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