改良培养基中海洋微藻小球藻脂肪酸的气相色谱-质谱联用分析

D. Kurnia
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引用次数: 3

摘要

微藻被称为制药工业的原料,它含有多种化合物,如蛋白质、脂肪酸、色素和维生素。该化合物可作为药物的基本成分,也可作为癌症和高胆固醇的治疗或预防剂。小球藻是一种生长在印度尼西亚海域的绿色微藻,它的脂质也足够高。对改性培养基培养的海洋微藻小球藻脂肪酸组成进行了分析。以10%转基因为培养基培养微藻8天。51,55 L培养产生31,0537 g干生物量。31,0537克干生物质Soxhletation产生约12,8818% w/w的原油。GC-MS分析结果显示,在小球藻中鉴定出3种脂肪酸,组成分别为棕榈酸(13.34%)、亚油酸(5.39%)和油酸(16.40%)。关键词:小球藻,脂肪酸,气相色谱-质谱法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
FATTY ACID ANALYSIS OF MARINE MICROALGAE Chlorella Sp. IN MODIFIED MEDIUM USED GAS CRHOMATOGRAPHY-MASS SPECTROMETRY (GC-MS)
Microalgae is known as a raw materials of pharmaceutical industry, it contains various chemical compounds such as protein, fatty acid, pigments and vitamins. The compound can be use as a basic ingredient of pharmaceutical also can be use as a treatment or prevention agent such as cancer and high cholesterol. Chlorella sp. is a green microalgae which can be found in Indonesia marine area, it also has a high enough of lipid. Compotition of fatty acid from marine microalgae Chlorella sp that cultivated with modification medium has been analyzed. Microalgae culture cultivated with GM 10% as a medium for 8 days. 51,55 L culture produced a 31,0537 gr dry biomass. 31,0537 gr dry biomass Soxhletation produced about 12,8818% w/w oil rendemen. The results of GC-MS analysis, there are 3 types of fatty acids identified in microalgae Chlorella Sp. The composition are palmitic acid (13,34%), linoleic acid (5,39%) and oleic acid (16,40%). Total fatty acid contained is 35,13% Keywords:Chlorella sp, fatty acids, GC-MS.
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