R. DEGUCHI, K. WATANABE, Y. KOGA, H. KIJIMA, A. TAKAGI
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Histological gastritis was evaluated using the updated Sydney system and scored from 0 to 3. Tyrosine phosphorylation was analysed using immunoprecipitation and immunoblotting.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Anti-CagA titres were correlated with the severity of mononuclear cell infiltration. In contrast, mean anti-CagA in patients with grade 2 bacterial density was significantly higher than those with grade 0 (<i>P</i> < 0.01). Mean anti-titre in patients with grade 3 tended to be lower than that seen with grade 2. CagA tyrosine phosphorylation was significantly more common in bacteria isolated from CagA-antibody-negative patients (93%;13/14) than from CagA-antibody-positive patients (29%; 4/14, <i>P</i> = 0.0007).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Host–bacteria interaction is regarded as one of the important factors in the development of gastroduodenal diseases.</p>\n </section>\n </div>","PeriodicalId":50822,"journal":{"name":"Alimentary Pharmacology & Therapeutics Symposium Series","volume":"2 1","pages":"127-131"},"PeriodicalIF":0.0000,"publicationDate":"2006-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1746-6342.2006.00035.x","citationCount":"1","resultStr":"{\"title\":\"Interaction between Helicobacter pylori and immune response to CagA: CagA antibody may down-regulate bacterial colonization and tyrosine phosphorylation\",\"authors\":\"R. DEGUCHI, K. WATANABE, Y. 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Histological gastritis was evaluated using the updated Sydney system and scored from 0 to 3. Tyrosine phosphorylation was analysed using immunoprecipitation and immunoblotting.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Anti-CagA titres were correlated with the severity of mononuclear cell infiltration. In contrast, mean anti-CagA in patients with grade 2 bacterial density was significantly higher than those with grade 0 (<i>P</i> < 0.01). Mean anti-titre in patients with grade 3 tended to be lower than that seen with grade 2. CagA tyrosine phosphorylation was significantly more common in bacteria isolated from CagA-antibody-negative patients (93%;13/14) than from CagA-antibody-positive patients (29%; 4/14, <i>P</i> = 0.0007).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>Host–bacteria interaction is regarded as one of the important factors in the development of gastroduodenal diseases.</p>\\n </section>\\n </div>\",\"PeriodicalId\":50822,\"journal\":{\"name\":\"Alimentary Pharmacology & Therapeutics Symposium Series\",\"volume\":\"2 1\",\"pages\":\"127-131\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1746-6342.2006.00035.x\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Alimentary Pharmacology & Therapeutics Symposium Series\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/j.1746-6342.2006.00035.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Alimentary Pharmacology & Therapeutics Symposium Series","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1746-6342.2006.00035.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
背景CagA+幽门螺杆菌菌株与胃十二指肠疾病的发生有关。幽门螺杆菌具有IV型分泌系统，负责将CagA转运到宿主细胞中。目的探讨CagA水平与组织学胃炎严重程度的关系，分析CagA酪氨酸磷酸化水平。方法采用尿幽门螺杆菌抗体、组织学检查和血清CagA抗体检测149例幽门螺杆菌感染情况。组织学胃炎采用更新的Sydney评分系统进行评分，评分范围从0到3。采用免疫沉淀和免疫印迹法分析酪氨酸磷酸化。结果抗caga滴度与单核细胞浸润程度相关。相比之下，2级细菌密度患者的平均抗caga明显高于0级细菌密度患者(P < 0.01)。3级患者的平均抗滴度往往低于2级患者。CagA酪氨酸磷酸化在CagA抗体阴性患者(93%;13/14)中比在CagA抗体阳性患者(29%;4/14, p = 0.0007)。结论宿主-细菌相互作用是胃十二指肠疾病发生发展的重要因素之一。
Interaction between Helicobacter pylori and immune response to CagA: CagA antibody may down-regulate bacterial colonization and tyrosine phosphorylation
Summary
Background
CagA+Helicobacter pylori strains are associated with the development of gastroduodenal diseases. H. pylori possess a type IV secretion system that is responsible for the translocation of CagA into host cells.
Aim
To investigate the correlation between the CagA status and the severity of histological gastritis and to analyse the tyrosine phosphorylation of CagA.
Methods
A total of 149 H. pylori status was determined via urine antibody to H. pylori, histological examination and serum antibody against CagA. Histological gastritis was evaluated using the updated Sydney system and scored from 0 to 3. Tyrosine phosphorylation was analysed using immunoprecipitation and immunoblotting.
Results
Anti-CagA titres were correlated with the severity of mononuclear cell infiltration. In contrast, mean anti-CagA in patients with grade 2 bacterial density was significantly higher than those with grade 0 (P < 0.01). Mean anti-titre in patients with grade 3 tended to be lower than that seen with grade 2. CagA tyrosine phosphorylation was significantly more common in bacteria isolated from CagA-antibody-negative patients (93%;13/14) than from CagA-antibody-positive patients (29%; 4/14, P = 0.0007).
Conclusions
Host–bacteria interaction is regarded as one of the important factors in the development of gastroduodenal diseases.
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