μ TAS声学侧阱的温度和俘获特性

TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference Pub Date : 2007-06-10 DOI:10.1109/SENSOR.2007.4300236

L. Johansson, M. Evander, T. Lilliehorn, M. Almqvist, J. Nilsson, T. Laurell, S. Johansson

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引用次数: 0

摘要

通过对μTAS(微全量分析系统)微流控通道中细胞或粒子的非接触捕获，对声学侧阱进行了温度和捕获效率测量。通过微通道中罗丹明B的荧光响应来测量温度。捕获效率是用被水动力平衡的捕获粒子簇的投影面积来衡量的。主要目标之一是获得流体通道中温度的定量值，以确保细胞和蛋白质的安全处理。其他目标是评估最佳驱动参数。在考虑温度和俘获的情况下，最优频率为平行共振频率。

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Temperature and Trapping Characterization of an Acoustic Lateral Trap for μ TAS

An acoustic lateral trap for application in non-contact trapping of cells or particles in a microfluidic channel in a μTAS (micro total analysis system) is characterized by temperature and trapping efficiency measurements. Temperature is measured by fluorescent response of Rhodamine B in the microchannel. Trapping efficiency is measured as the projected area of a trapped particle cluster counter-balanced by hydrodynamic force. One of the main objectives is to obtain quantitative values of the temperature in the fluidic channel to ensure safe handling of cells and proteins. Other objectives are to evaluate optimal drive parameters. The optimal frequency, when temperature and trapping is considered, was found to be the parallel resonance frequency.

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TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference

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