Gbemisola A. Adegoke , Samuel A. Onasanwo , O. David Eyarefe , Samuel B. Olaleye
{"title":"麝香皮提取物对大鼠醋酸性结肠炎的改善作用","authors":"Gbemisola A. Adegoke , Samuel A. Onasanwo , O. David Eyarefe , Samuel B. Olaleye","doi":"10.1016/j.jobaz.2016.06.004","DOIUrl":null,"url":null,"abstract":"<div><p>Ameliorative effects of <em>Musa sapientum</em> peel extract (MSPE) were studied on Acetic Acid (AA)-induced colitis in rats. From a dose response study, the effective and yet, safe for the treatment of AA-induced colitis in rats was determined, and also, a further study was carried out to determine the effective fraction(s) of MSPE that will be useful in the treatment of AA induced colitis in rats. Colitis was induced with 0.2<!--> <!-->ml of 6% acetic acid through a lower abdominal laparotomy. Treatment with graded doses of methanolic extract of dried peel of <em>Musa sapientum</em> (MS) (50, 100 and 200<!--> <!-->mg/kg) for 7<!--> <!-->days in AA induced colitis in rats showed 50<!--> <!-->mg/kg as an optimal effective dose for the healing of AA induced colitis in rats. This dose (50<!--> <!-->mg/kg) was further studied with fractions of MSPE in comparison to a standard drug (sulfasalazine) of the same dose (50<!--> <!-->mg/kg) on AA induced colitis in rats for 18<!--> <!-->days. Colitis was assessed using stool consistency, macroscopic gross score and histological studies. Normal stool consistency was seen early in treated animals as compared with control; MS peel (crude extracts and fractions) significantly reduced macroscopic and histologic colon tissue damage in a manner similar to that of sulfasalazine. The results from these findings suggests that MSPE fractions of ethyl acetate and methanol may be effective in reducing both macroscopic and histological damage in a manner similar to sulfasalazine, and that it may be helpful in the treatment of colitis.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"77 ","pages":"Pages 49-55"},"PeriodicalIF":1.1000,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.06.004","citationCount":"5","resultStr":"{\"title\":\"Ameliorative effects of Musa sapientum peel extract on acetic acid-induced colitis in rats\",\"authors\":\"Gbemisola A. Adegoke , Samuel A. Onasanwo , O. David Eyarefe , Samuel B. Olaleye\",\"doi\":\"10.1016/j.jobaz.2016.06.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Ameliorative effects of <em>Musa sapientum</em> peel extract (MSPE) were studied on Acetic Acid (AA)-induced colitis in rats. From a dose response study, the effective and yet, safe for the treatment of AA-induced colitis in rats was determined, and also, a further study was carried out to determine the effective fraction(s) of MSPE that will be useful in the treatment of AA induced colitis in rats. Colitis was induced with 0.2<!--> <!-->ml of 6% acetic acid through a lower abdominal laparotomy. Treatment with graded doses of methanolic extract of dried peel of <em>Musa sapientum</em> (MS) (50, 100 and 200<!--> <!-->mg/kg) for 7<!--> <!-->days in AA induced colitis in rats showed 50<!--> <!-->mg/kg as an optimal effective dose for the healing of AA induced colitis in rats. This dose (50<!--> <!-->mg/kg) was further studied with fractions of MSPE in comparison to a standard drug (sulfasalazine) of the same dose (50<!--> <!-->mg/kg) on AA induced colitis in rats for 18<!--> <!-->days. Colitis was assessed using stool consistency, macroscopic gross score and histological studies. Normal stool consistency was seen early in treated animals as compared with control; MS peel (crude extracts and fractions) significantly reduced macroscopic and histologic colon tissue damage in a manner similar to that of sulfasalazine. The results from these findings suggests that MSPE fractions of ethyl acetate and methanol may be effective in reducing both macroscopic and histological damage in a manner similar to sulfasalazine, and that it may be helpful in the treatment of colitis.</p></div>\",\"PeriodicalId\":31288,\"journal\":{\"name\":\"Journal of Basic and Applied Zoology\",\"volume\":\"77 \",\"pages\":\"Pages 49-55\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2016-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.06.004\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Basic and Applied Zoology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2090989616300273\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Basic and Applied Zoology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2090989616300273","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 5
摘要
研究了麝香皮提取物(MSPE)对醋酸(AA)诱导大鼠结肠炎的改善作用。通过剂量反应研究,确定了治疗AA性大鼠结肠炎的有效且安全的方法,并进一步研究了MSPE治疗AA性大鼠结肠炎的有效组分。下腹部剖腹术用0.2 ml 6%乙酸诱导结肠炎。AA性结肠炎大鼠按不同剂量(50、100和200 mg/kg)给药7 d,结果表明50 mg/kg是AA性结肠炎大鼠愈合的最佳有效剂量。进一步研究了该剂量(50 mg/kg)与相同剂量(50 mg/kg)的标准药物(柳氮磺胺吡啶)对AA性结肠炎大鼠18天的作用。结肠炎的评估采用粪便一致性、宏观评分和组织学研究。与对照组相比,治疗动物的粪便粘稠度较早出现正常;MS果皮(粗提取物和馏分)显著减少宏观和组织学上的结肠组织损伤,其方式与柳氮磺胺吡啶相似。这些发现的结果表明,MSPE乙酸乙酯和甲醇的组分可能有效地减少宏观和组织学损伤,其方式类似于磺胺吡啶,并且可能有助于治疗结肠炎。
Ameliorative effects of Musa sapientum peel extract on acetic acid-induced colitis in rats
Ameliorative effects of Musa sapientum peel extract (MSPE) were studied on Acetic Acid (AA)-induced colitis in rats. From a dose response study, the effective and yet, safe for the treatment of AA-induced colitis in rats was determined, and also, a further study was carried out to determine the effective fraction(s) of MSPE that will be useful in the treatment of AA induced colitis in rats. Colitis was induced with 0.2 ml of 6% acetic acid through a lower abdominal laparotomy. Treatment with graded doses of methanolic extract of dried peel of Musa sapientum (MS) (50, 100 and 200 mg/kg) for 7 days in AA induced colitis in rats showed 50 mg/kg as an optimal effective dose for the healing of AA induced colitis in rats. This dose (50 mg/kg) was further studied with fractions of MSPE in comparison to a standard drug (sulfasalazine) of the same dose (50 mg/kg) on AA induced colitis in rats for 18 days. Colitis was assessed using stool consistency, macroscopic gross score and histological studies. Normal stool consistency was seen early in treated animals as compared with control; MS peel (crude extracts and fractions) significantly reduced macroscopic and histologic colon tissue damage in a manner similar to that of sulfasalazine. The results from these findings suggests that MSPE fractions of ethyl acetate and methanol may be effective in reducing both macroscopic and histological damage in a manner similar to sulfasalazine, and that it may be helpful in the treatment of colitis.