Culture medium, extracellular components and differentiation in culturing of human pluripotent stem cells

The cultivation of human pluripotent stem cells (hPSCs) has become an important factor in regenerative medicine and the development of drugs. Similar to other mammalian cells, human pluripotent stem cells are important in culture for the formation of microenvironment, including intercellular interactions, growth factors, and contact between cells and matrix [1]. The culture of stem cells is similar to that of standard mammalian cell culture, but differentiation or maintenance of undifferentiated state may be necessary for the purpose. Phenotypic characteristics in pluripotent stem cells (morphology, colony assessment, pluripotent potential) and in differentiated cells (morphology, differentiation markers, functionality) should be evaluated [1].