标记辅助选择绿豆金字塔系抗白粉病及农艺性能评价

IF 0.6 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Ketwalee Siwapithakpong, W. Chueakhunthod, A. Khairum, A. Tharapreuksapong, Theerawat Chantakot, Tanandorn Woramit, P. Tantasawat
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引用次数: 0

摘要

基因金字塔是绿豆提供持久和高水平白粉病抗性的有效策略[Vigna radiata (L.)]。r . Wilczek]。本研究的目的是通过标记辅助选择KING(复发亲本)× SUPER5(供体亲本)杂交获得的BC2F1锥体后代对PM的抗性水平,并在田间条件下测量其农艺性能。结果表明,I85420 + I42PL222和I27R565这两个抗PM基因标记组合的后代对PM的抗性(平均疾病严重程度评分为3.80)高于KING,检查cv。无标记组合的子代得分分别为8、8和6.75。而仅携带1个抗性基因的后代则含有I85420 + I42PL222或I27R565,反应得分为5.83分。此外,大多数金字塔系的单株产量都高于KING系,因为它们的单株荚果、单株簇和单荚种子都优于KING系。未来,这些具有两个抗PM基因的金字塔系可以通过标记辅助回交育种进一步培育出新的PM抗性品种,或作为绿豆育种计划中潜在的PM抗性来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Powdery Mildew Resistance and Agronomic Performance of Pyramided Mungbean Lines from Marker- Assisted Selection
Gene pyramiding is an effective strategy to provide a durable and high level of powdery mildew (PM) resistance in mungbean [Vigna radiata (L.) R. Wilczek]. The objectives of this study were to evaluate PM resistance levels of pyramided BC2F1 progenies derived from marker-assisted selection of KING (recurrent parent) × SUPER5 (donor parent) cross and measure their agronomic performance under fi eld condition. The results showed that the progenies with marker combinations linked to both PM resistance genes including I85420 + I42PL222, and I27R565 were more resistant to PM (average disease severity score of 3.80) than KING, check cv. CN72, and the progenies without any marker combinations (scores of 8, 8 and 6.75, respectively). While the progenies carrying only one resistance gene were identifi ed as containing either I85420 + I42PL222, or I27R565 which showed intermediate response score of 5.83. In addition, most of the pyramided lines produced higher yield per plant than KING because of their superior pods per plant, clusters per plant, and seeds per pod. In the future, these pyramided lines with two PM resistance genes can be further developed into new PM resistant varieties via marker-assisted backcross breeding or be used as potential PM resistant sources in mungbean breeding programs.
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来源期刊
Chiang Mai Journal of Science
Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.00
自引率
25.00%
发文量
103
审稿时长
3 months
期刊介绍: The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
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