用EST-SSR标记分析大花蕙兰及其亲缘种质的分化及亲缘关系

Meiyu Li, Qing Luo, Xueying Zhao, Changchun Ding, Zhaojin Chi, Zuzheng Lu, Wenhao Bo
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引用次数: 0

摘要

基于分子标记的兰科植物种、属、杂交种开花前鉴定具有重要的育种和遗传研究价值。本研究选择大花蕙兰(Cymbidium tortisepalum)种质‘Lianbansu’进行转录组测序,从28,235个单基因中发现9,485个推测的EST-SSRs。然后从设计的引物中随机抽取192对引物,通过实验筛选出38对优质引物。利用这些高质量标记对51份兰花种质资源的遗传多样性、群体结构和进化历史进行了分析。等位基因数为3 ~ 12个,平均为6个。观察到的杂合度和期望杂合度的平均值分别为0.4662和0.5973。前两个主坐标检测到39.66%的总变异。发现ΔK-value有一个峰值(当K = 2时)。利用这些标记可以有效地区分金花蕙兰(C. tortisepalum)、中国蕙兰(Cymbidium sinense)和大花蕙兰(Cymbidium hybridum)的几种种质,以及一些种间或属间杂种和未鉴定的种质。总之,本研究建立了一套具有较强可转移性的EST-SSR标记,可用于兰花花前杂交亲本追踪和种质鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Divergence and relationships of Cymbidium tortisepalum and its kindred germplasms by EST-SSR markers
ABSTRACT Molecular marker-based identification of Chinese orchids among species, genera, and hybrids before flowering has important breeding and genetic research value. In this study, the Cymbidium tortisepalum germplasm ‘Lianbansu’ was chosen for transcriptome sequencing, yielding 9,485 putative EST-SSRs discovered from 28,235 unigenes. Then, 192 primers were randomly selected from the designed primers, and 38 pairs of high-quality primers were screened out through experiments. These high-quality markers were used to examine the genetic diversity, population structure, and evolutionary history of 51 orchid germplasms. The number of alleles ranged from 3 to 12, with an average of 6. The observed and expected heterozygosity with an average of 0.4662 and 0.5973. The first two principal coordinates detected 39.66% of the total variation. It was found that the ΔK-value had one peak (When K = 2). Several germplasms of C. tortisepalum, Cymbidium sinense, Cymbidium hybridum, and some interspecific or intergeneric hybrids and unidentified germplasms could be effectively distinguished using these markers. In conclusion, a set of EST-SSR markers with outstanding transferability was created in this study that can be utilised for hybrid parent tracing and germplasm identification of orchids before flowering.
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