In Vitro Growth of Nyamplung (Callophylum inophyllum): The Future Generation Biofule Plants

Nyamplung (Calophyllum inophyllum L.) is a potential tree for renewable world energy resources, producing future generation biofuel plants. Propagation technique is required for large scale plantation with highest oil yield of nyamplung. In this study, tissue culture techniques were used for evaluated the optimized nyamplung growth hormone. Shoots rejuvenation were used as explant in MS media enriched with NAA 0.5 mgL⁻¹, 1 mgL⁻¹; 1.5 mgL⁻¹; BAP 1 mgL⁻¹, 2 mgL⁻¹ and 3 mgL⁻¹ and Kinetin 0.15 mgL⁻¹. Shoot and root were induced on the all media used after 10 months incubation. The highest shoot elongation was MS media enriched with 0.5 mgL⁻¹ NAA, 1 mgL⁻¹ BAP and 0.15 mgL⁻¹ Kinetin (10.54 ± 1.98 cm) and the highest root elongation was also in this media (4.65 ± 0.68 cm). Although all the explants rooted after 3 months, in this same media induced a faster root differentiation than the other treatments. NAA concentration higher than 0.5 mgL⁻¹ had no effect on the sprouting process. BAP concentration higher than 3 mgL⁻¹ inhibited callus inductions. The low rates of mortality of rooted plantlets were observed in acclimatization (98 % plants live and grow well up to 6 months of observation). The best hormone concentration of nyamplung media regeneration was expected the highest multiplication for mass micro propagation.