山羊亚临床乳腺炎(Capra hircus)乳铁蛋白基因的分子特征及相关性研究

Q3 Agricultural and Biological Sciences
Y. O. Waminal, G. A. S. Tubalinal, C. Mingala
{"title":"山羊亚临床乳腺炎(Capra hircus)乳铁蛋白基因的分子特征及相关性研究","authors":"Y. O. Waminal, G. A. S. Tubalinal, C. Mingala","doi":"10.4025/actascianimsci.v44i1.56368","DOIUrl":null,"url":null,"abstract":"The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus)\",\"authors\":\"Y. O. Waminal, G. A. S. Tubalinal, C. Mingala\",\"doi\":\"10.4025/actascianimsci.v44i1.56368\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme.\",\"PeriodicalId\":7149,\"journal\":{\"name\":\"Acta Scientiarum. Animal Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Scientiarum. Animal Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4025/actascianimsci.v44i1.56368\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Scientiarum. Animal Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4025/actascianimsci.v44i1.56368","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

摘要

该研究表征了菲律宾不同山羊品种的乳铁蛋白(Lf) mRNA基因,并确定了其与亚临床乳腺炎(SCM)的关系。本研究收集哺乳第二周的乳汁(n=75)和血液样本(n=5),提取RNA,利用cDNA通过聚合酶链反应扩增Lf基因。测定其核苷酸和氨基酸序列,作为评价系统发育关系的参考。利用扩增产物进行RFLP分析,确定该基因与SCM的相关性。结果表明,山羊的Lf基因分子量为2135。Saanen、ang鲁- nubian和菲律宾本地山羊的Lf基因核苷酸和氨基酸序列与提交GenBank的Capra hircus (U53857)的Lf基因高度相似(99%)。系统发育研究表明,盎格鲁-努比亚山羊、萨南山羊和本地山羊的Lf基因与C. hircus (U53857)的Lf基因是同源的。利用限制性内切酶AluI和HaeIII记录了山羊的三种基因型。利用HaeIII限制性内切酶对BB型山羊的Lf基因与SCM的相关性进行了统计分析(p = 0.0308, p = 5.65)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus)
The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Acta Scientiarum. Animal Sciences
Acta Scientiarum. Animal Sciences Agricultural and Biological Sciences-Food Science
CiteScore
1.60
自引率
0.00%
发文量
45
审稿时长
9 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信