MeDIP-seq揭示中华绒螯蟹未成熟睾丸线粒体基因组甲基化特征

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY

Mitochondrial Dna Part a Pub Date : 2018-04-01 DOI:10.1080/24701394.2016.1278537

Gen-Liang Li, Yi-Jiao Xu, Xiaomin Huang, Juan Xiao, Song Nong, Chao-gan Li

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引用次数: 3

摘要

摘要本研究首次利用MeDIP-seq技术对长江水系中华绒螯蟹(Eriocheir sinensis)未成熟睾丸线粒体基因组的甲基化进行了研究。我们的甲基化DNA片段覆盖了从GenBank加载的中华鄂蚊线粒体基因组的99%以上。螃蟹线粒体基因组有8个突变碱基和42个snp。甲基化出现在所有基因以及A + T区域，但在线粒体基因组的基因间区域较少。然而，大多数编码蛋白质的基因和A + T区甲基化水平较高。但是，大多数编码trna的基因是低甲基化的，两种rRNA基因也显示出低甲基化或中甲基化的频率。尤其是基因间区甲基化水平最低。这些特征表明，DNA甲基化可能在中华绒螯蟹未成熟睾丸线粒体基因组基因表达调控中起重要作用。

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MeDIP-seq reveals the features of mitochondrial genomic methylation in immature testis of Chinese mitten crab Eriocheir sinensis

Abstract In this study, the methylation of mitochondrial genome in the immature testis of Chinese mitten crab Eriocheir sinensis of the Yangtze River system was determined for the first time using MeDIP-seq. Our methylated DNA fragments covered more than 99% of the mitochondrial genome in E. sinensis loaded from GenBank. There were 8 mutated bases and 42 SNPs in the crab mitochondrial genome. The methylation presented in all genes as well as in an A + T region, but less in intergenic regions in the mitochondrial genome. However, the level of methylation of most genes coding proteins and the A + T region were high. But, the majority of genes encoding tRNAs were hypomethylated, and both the rRNA genes also showed methylation of low or median frequency. Especially, the level of methylation of the intergenic regions is the lowest. Those features indicated that the methylation of DNA may play an important role in gene expressing regulation in the mitochondrial genome of immature testis in E. sinensis.

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.