用辣木叶提取物强化保存培养基提高冷藏和冷冻保存雄鹿精子的能力

M. Khalifa
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摘要

本试验研究了在雄精子培养基中添加辣木叶提取物对雄精子液冷保存和精子冻存能力的影响。在9 - 10月期间,用人工阴道收集了5只大马士革雄鹿的20次射精,每只4次射精。每个采集阶段的混合标本用三柠檬黄蛋黄扩展剂稀释(1:10),分成6等份。第1组为对照(不含辣木),其余5组分别添加0.05、0.1、0.2、0.3、0.4 mL/ mL (v/v)辣木叶提取物。然后将样品保存并在4℃下保存48小时,在此期间评估精子性状以及丙二醛(MDA)产生和谷胱甘肽过氧化物酶(GPX)活性。进一步研究了辣木叶提取物的最佳浓度,发现辣木叶提取物在整个冷藏期间保持精子性状的潜力,以保持精子在低温保存胁迫下的寿命。结果表明:添加辣木叶提取物降低了精子氧化应激(P<0.05),与精子活力(%)、活力(%)、正常精子(%)、顶体完整性和GPX活性呈正相关(P<0.01) (r分别为0.36、0.42、0.61、0.79和0.35),与丙二醛(MDA)生成呈负相关(P<0.01) (r= - 0.56)。与对照组相比,在雄精子培养基中添加0.4 mL/ mL辣木叶提取物能有效改善(P<0.05)雄精子的各项性状,降低(P<0.05)雄精子解冻后DNA断裂指数(DFI)。这些结果表明,辣木叶提取物作为雄精子培养基中外源抗氧化剂的补充具有强大的潜力,特别是当精子用于人工授精和体外受精应用时。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Improving Capacities of Chilled- and Cryo-Preserved Buck Spermatozoa by Enriching Preservation Medium with Moringa oleifera leaves extract
In the present work, two experiments were conducted to investigate the efficiency of supplementing buck sperm medium with Moringa oleifera leaves extract on liquid-chilled storage and cryopreservation capacities of spermatozoa. Twenty ejaculates were collected by an artificial vagina from 5 Damascus bucks, 4 ejaculates each, during September-October. The pooled specimens of each collection session were diluted (1:10) with Tris-citric egg yolk extender, and were split into 6 equal aliquots. The first aliquot served as control (moringa-free), whereas the other five aliquots were supplemented with 0.05, 0.1, 0.2, 0.3 or 0.4 mL/ml (v/v) moringa leaves extract, respectively. The samples were then stored and maintained at 4 oC for the subsequent 48 h during which sperm traits were assessed alongside malondialdehyde (MDA) production and glutathione peroxidase (GPX) activity. The optimum concentration of moringa leaves extract that showed potential for maintaining sperm traits throughout the chilled storage period was further investigated for preserving the lifespan of spermatozoa after exposure to the cryopreservation stress against control. The results showed that moringa leaves extract supplementation reduced (P<0.05) the oxidative stress on spermatozoa and recorded a positive correlation (P<0.01) with sperm motility (%), viability (%), normal sperm (%), acrosome integrity and GPX activity (r= 0.36, 0.42, 0.61, 0.79 and 0.35, respectively) while recording a negative correlation (P<0.01) with production of MDA (r= - 0.56). Furthermore, supplementing buck sperm medium with 0.4 mL/ml moringa leaves extract efficiently improved (P<0.05) all sperm characteristics and decreased (P<0.05) DNA fragmentation index (DFI) post thawing compared to those of control. These results imply the powerful potential of moringa leaves extract as an exogenous antioxidant supplement in buck sperm medium particularly when spermatozoa are used for AI and IVFM applications.
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