蛋白酶水解丝素蛋白支架的研究

P. Srihanam, W. Simchuer
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引用次数: 9

摘要

利用家蚕直接在玻璃板上纺出二维丝支架,并利用蛋白酶XXIII进行蛋白水解降解研究。酶液37℃孵育10周。研究了支架的表面形貌、二级结构、质量损失、降解率和粒径等性能。蛋白酶作用后丝素蛋白支架结构发生轻微变化。在酶孵育期结束时,它们的质量也大于20%。在4周的时间内,支架的降解速度很快,颗粒大小在1 ~ 15 μm之间出现了散射。结果表明,蛋白酶XXIII是一种能够降解丝素蛋白的酶。因此,该酶可作为模型研究或用于制备蚕丝材料,进一步推广应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Proteolytic Degradation of Silk Fibroin Scaffold by Protease XXIII
Two-dimensional silk scaffolds were directly spun on the glass plates by Bombyx mori (called locally Nang- Lai) silkworms and used as subject for proteolytic degradation study with protease XXIII. The silk scaffolds were incu- bated with enzyme solution at 37 °C up to 10 weeks. Those of the scaffold properties: surface morphology, secondary structure, mass loss, degradation ratios and particle sizes were investigated. Silk fibroin scaffolds slightly changed of their structure after exposure to the protease. They were also loose of mass greater than 20% at the end of enzyme incubation periods. Much degradation of the scaffolds was quickly during the 4 weeks and scattering of the particle sizes between 1 to 15 μm were found. The results suggested that protease XXIII is an enzyme which could be degraded of the silk fibroin. Therefore, it will be used this enzyme as model study or for preparing silk material for further applications.
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