纳米银Er3+/Yb3+共掺碲酸钨玻璃的荧光成像

S. K. Mahajan, R. Jain
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引用次数: 0

摘要

采用熔体淬火技术合成了Er3+ - yb3 +掺杂TeO2 - wo3 - Li2O (TWLEY)三元掺杂和不掺杂Ag纳米玻璃。利用共聚焦荧光显微镜λ扫描技术,在488nm激光激发下,测量并验证了玻璃表面等离子体共振带在535 ~ 550 nm和640 ~ 650 nm范围内的分布。在545nm处观察TWLEY和TWLEY03A的荧光强度图。利用PicoQuant公司405nm、6ns、40mhz的脉冲激光二极管对荧光寿命成像显微镜(FLIM)进行了成像和寿命测量研究,并对结果进行了衰减和寿命直方图分析。该研究表明,等离子体激元增强了银和稀土掺杂玻璃的光致发光,可用于生物医学成像。采用熔体淬火技术合成了Er3+ - yb3 +掺杂TeO2 - wo3 - Li2O (TWLEY)三元掺杂和不掺杂Ag纳米玻璃。利用共聚焦荧光显微镜λ扫描技术,在488nm激光激发下,测量并验证了玻璃表面等离子体共振带在535 ~ 550 nm和640 ~ 650 nm范围内的分布。在545nm处观察TWLEY和TWLEY03A的荧光强度图。利用PicoQuant公司405nm、6ns、40mhz的脉冲激光二极管对荧光寿命成像显微镜(FLIM)进行了成像和寿命测量研究,并对结果进行了衰减和寿命直方图分析。该研究表明,等离子体激元增强了银和稀土掺杂玻璃的光致发光,可用于生物医学成像。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fluorescence imaging of Er3+/Yb3+ codoped tungsten tellurite glasses with silver nanoparticles
The Er3+ - Yb 3+ doped TeO2 -WO3- Li2O (TWLEY) ternary doped with and without Ag nanoparticles (NPs) glasses have been synthesized by melt quenching technique. Surface Plasmon resonance bands in the glasses are measured and verified in the range 535-550 nm and 640-650 nm under 488nm laser excitation by lambda scanning technique of the confocal fluorescence microscope. Fluorescence Intensity map of TWLEY and TWLEY03A are observed at 545nm. The Fluorescence Lifetime Imaging Microscopy (FLIM) studies has been conducted for the imaging and lifetime measurement using pulsed laser diode of 405 nm, 6ns,40 MHz of PicoQuant and result are analyzed by decay and lifetime histograms. This work shows that Plasmon enhances photoluminescence of silver and rare-earth doped glasses may be utilized for biomedical imaging.The Er3+ - Yb 3+ doped TeO2 -WO3- Li2O (TWLEY) ternary doped with and without Ag nanoparticles (NPs) glasses have been synthesized by melt quenching technique. Surface Plasmon resonance bands in the glasses are measured and verified in the range 535-550 nm and 640-650 nm under 488nm laser excitation by lambda scanning technique of the confocal fluorescence microscope. Fluorescence Intensity map of TWLEY and TWLEY03A are observed at 545nm. The Fluorescence Lifetime Imaging Microscopy (FLIM) studies has been conducted for the imaging and lifetime measurement using pulsed laser diode of 405 nm, 6ns,40 MHz of PicoQuant and result are analyzed by decay and lifetime histograms. This work shows that Plasmon enhances photoluminescence of silver and rare-earth doped glasses may be utilized for biomedical imaging.
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