S. Tripathy, P. Mohanty, M. Jena, G. Dash, K. Pradhan, P. Nayak, S. Dash, D. Lenka, D. Mishra, P. Mohapatra, D. Swain, N. Senapati
{"title":"豇豆(Vigna mungo (L.))遗传变异对比揭示及遗传多样性研究Hepper)利用SDS-PAGE分析种子贮藏蛋白","authors":"S. Tripathy, P. Mohanty, M. Jena, G. Dash, K. Pradhan, P. Nayak, S. Dash, D. Lenka, D. Mishra, P. Mohapatra, D. Swain, N. Senapati","doi":"10.19071/RIB.2016.V7.2863","DOIUrl":null,"url":null,"abstract":"Total seed storage protein profiles of 20 urdbean genotypes including the popular variety T9 were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 14 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into three protein types. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 78.5% phenon level. TU 95-1 with TU 12-25-4 revealed lowest similarity index value (0.33) followed by TU 95-1 with PU 30 and KU 96-3(SI=0.35). Clustering pattern revealed distinctly divergent group formed by TPU 95-1 and TPU 4. These may serve as a valuable source genotype in recombination breeding. Key words: Seed storage protein profiling, SDS-PAGE, Genetic variation, urdbean.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"47 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2016-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Revealing contrasting genetic variation and study of genetic diversity in urdbean (Vigna mungo (L.) Hepper) using SDS-PAGE of seed storage proteins\",\"authors\":\"S. Tripathy, P. Mohanty, M. Jena, G. Dash, K. Pradhan, P. Nayak, S. Dash, D. Lenka, D. Mishra, P. Mohapatra, D. Swain, N. Senapati\",\"doi\":\"10.19071/RIB.2016.V7.2863\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Total seed storage protein profiles of 20 urdbean genotypes including the popular variety T9 were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 14 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into three protein types. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 78.5% phenon level. TU 95-1 with TU 12-25-4 revealed lowest similarity index value (0.33) followed by TU 95-1 with PU 30 and KU 96-3(SI=0.35). Clustering pattern revealed distinctly divergent group formed by TPU 95-1 and TPU 4. These may serve as a valuable source genotype in recombination breeding. Key words: Seed storage protein profiling, SDS-PAGE, Genetic variation, urdbean.\",\"PeriodicalId\":21082,\"journal\":{\"name\":\"Research in Biotechnology\",\"volume\":\"47 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-02-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research in Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.19071/RIB.2016.V7.2863\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.19071/RIB.2016.V7.2863","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Revealing contrasting genetic variation and study of genetic diversity in urdbean (Vigna mungo (L.) Hepper) using SDS-PAGE of seed storage proteins
Total seed storage protein profiles of 20 urdbean genotypes including the popular variety T9 were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 14 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into three protein types. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 78.5% phenon level. TU 95-1 with TU 12-25-4 revealed lowest similarity index value (0.33) followed by TU 95-1 with PU 30 and KU 96-3(SI=0.35). Clustering pattern revealed distinctly divergent group formed by TPU 95-1 and TPU 4. These may serve as a valuable source genotype in recombination breeding. Key words: Seed storage protein profiling, SDS-PAGE, Genetic variation, urdbean.
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