饲粮中添加锌对韩国地方鸡抗氧化指标及锌转运基因表达的影响

Dong-Gyung Jeon, Min-Jeong Kim, I. Yoon, Ho-Sung Ahn, S. Sohn, I. Jang
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引用次数: 0

摘要

选用4周龄雄性韩国土鸡(KNC),分为3组,每组6个重复(8只/重复):分别饲喂基础饲粮(CON, 100 ppm锌)、基础饲粮中添加50 ppm锌和氧化锌(ZnO)、基础饲粮中添加50 ppm锌和蛋氨酸锌(ZnM)。饲喂4周后,每组6只鸡,观察锌对抗氧化指标和锌转运基因mRNA表达的影响。血氮成分、脂质过氧化和总抗氧化状态不受加锌日粮的影响。与氧化锌组相比,锌纳米组尿酸水平显著升高(P<0.05)。锌对小肠超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)活性及丙二醛(MDA)水平无显著影响。添加锌-蛋氨酸显著(P<0.05)提高了谷胱甘肽s -转移酶(GST)活性。在肝脏中,添加锌-蛋氨酸可显著提高GST活性(P<0.05),但不影响SOD、GPX和MDA水平。在锌转运基因mRNA表达方面,与CON组相比,ZnM组肠道ZnT-1 (P=0.09)和ZnT-5 (P=0.06)水平有明显升高趋势。与CON组相比,ZnM组肝脏金属硫蛋白mRNA有上调的趋势(P=0.10)。综上所述,饲粮中添加50 ppm的锌蛋氨酸有助于提高KNC小肠和肝脏中GST活性和锌转运基因表达。(
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Dietary Zinc Supplements on the Antioxidant Indicators and the Expression of Zinc Transport Genes in Korean Native Chicks
Four-week-old male Korean native chicks (KNC) were assigned to 3 groups with 6 replicates (8 birds/replicate) in each group: a basal diet (CON, 100 ppm of Zn), basal diet fortified with 50 ppm of Zn with zinc oxide (ZnO), or basal diet fortified with 50 ppm of Zn with Zn-methionine (ZnM). Immediately after a 4-week-feeding trial, 6 birds per group were used to evaluate the effects of zinc supplements on antioxidant indicators and the mRNA expression of zinc transport genes. The nitrogen components, lipid peroxidation, and total antioxidant status in blood were not influenced by Zn fortified diets. However, the ZnM group showed a significant (P<0.05) increase in uric acid levels than those in the ZnO group. In the small intestine, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities, and malondialdehyde (MDA) level were unaffected by zinc supplements. The activity of glutathione S-transferase (GST) was significantly (P<0.05) enhanced by Zn-methionine supplementation. In the liver, the activity of GST was significantly (P<0.05) increased by Zn-methionine supplement without affecting SOD, GPX, and MDA levels. With respect to the mRNA expression of zinc transport genes, the ZnM group displayed a strong tendency for increases in intestinal ZnT-1 (P=0.09) and ZnT-5 (P=0.06) levels, compared to those in the CON group. Moreover, the ZnM group showed a tendency (P=0.10) for up-regulation of hepatic metallothionein mRNA as compared with the CON group. In conclusion, the Zn-fortified diet with 50 ppm of Zn-methionine helped to improve GST activity and Zn transport gene expression in the small intestine or liver of KNC. (
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