K. Eguchi, Emiko Oguri, Takanori Sasaki, A. Matsuo, D. Nguyen, W. Jaitrong, B. E. Yahya, Zhilin Chen, R. Satria, Wendy Y. Wang, Y. Suyama
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Revisiting museum collections in the genomic era: potential of MIG-seq for retrieving phylogenetic information from aged minute dry specimens of ants (Hymenoptera: Formicidae) and other small organisms
Multiplexed inter-simple sequence repeat genotyping by sequencing – MIG-seq – is an effective PCR-based method for genome-wide SNP detection using the Next-Generation Sequencing platform, and it provides a potential solution to a central problem in museomics – the difficulties of obtaining useful sequence data from aged specimens with often degraded and / or low yields of DNA. We demonstrate and validate the cost effectiveness and utility of the MIG-seq workflow in obtaining useful and robust sequence data from aged museum specimens. We applied the MIG-seq approach to 55 aged (10 23 years old) millimeter-sized dry-mounted specimens of the hyper-diverse ant genus Pheidole. A total of 50,782,736 reads were generated from the 55 samples (259,902 3,693,375 reads per sample). The reads corresponded to 36,862 SNPs from 4,849 polymorphic loci; the SNP dataset was then used to construct a Bayesian phylogenetic tree. The topology of the phylogenetic tree was highly compatible with existing knowledge of phylogenetic relationships among species of Pheidole. Therefore, we recommend the MIG-seq method as a cost-effective and highly applicable pipeline for conducting phylogenetic and population genetic studies on aged museum specimens, potentially enhancing the relevance of specimen repositories in general towards modern biodiversity science and conservation biology.
期刊介绍:
Taxonomic manuscripts with isolated species descriptions are generally discouraged, especially for genera with large numbers of undescribed species; it lies at the discretion of the editorial team whether such manuscripts are considered. Papers on new distribution records will be considered if the new records are sufficiently important or unexpected from a biogeographical perspective. Such papers could, for example, discuss relevant biological/ecological data and/or biogeographical implications such as analysis by species-distribution modelling.
In detail, research areas covered by Myrmecological News include: behaviour; biogeography and faunistics; biological-pest control; chemical ecology; climate-change biology; cognition and learning; comparative and functional morphology; community ecology; conservation biology and bioindication; cytogenetics; ecology and evolution of (endo)symbioses; ecosystem (dis)services; foraging strategies; fossils; fragmentation ecology; genomics; histology; immune research; (integrative) taxonomy; interspecific hybridisation; invasion biology; life-history research; methodology in community quantification; national checklists; neurobiology; niche ecology; orientation and navigation; phenology; phylogeny and phylogeography; population genetics; sensory physiology; social evolution; social parasitism; sociogenomics; stable isotopes; tools for routine identification.