固定化和共固定化微球吸附柠檬烯和葡萄糖的动力学研究

A. Damayanti, S. Sarto, W. B. Sediawan
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引用次数: 0

摘要

腐烂的橙子含有葡萄糖和柠檬烯,其中柠檬烯是微生物的抑制剂。用包埋法固定混合培养物是保护混合培养物不受抑制剂侵害的最简单方法。挤压滴注包埋法是制备微珠的一种高效技术。本研究旨在测定吸附物(葡萄糖和柠檬烯)在吸附剂表面(微珠)的吸附速率。本研究使用的材料为葡萄糖、dl -柠檬烯、混合培养物和微球。三种微球类型分别为海藻酸盐-无混合培养(A)、海藻酸盐-活性炭-无混合培养(CA)、海藻酸盐-无活性炭混合培养(CB)。吸附柱由30毫升营养物质,15毫升底物和5毫升微珠组成。如果微珠不含混合培养物,则将营养物和基质替换为aquadest。反应器在37℃的间歇系统中进行。对葡萄糖的吸附能力依次为AG、CAG和CBG,对柠檬烯溶液的吸附能力依次为AL、CBL和CAL。采用拉格伦模型测定了其对柠檬烯和葡萄糖的吸附动力学。葡萄糖溶液的准二级吸附速率(k2,ad)在0.025 ~ 0.087 min-1之间,d -柠檬烯在2.084 ~ 5.233 min-1之间。三种吸附剂对葡萄糖和柠檬烯的吸附在60分钟时达到稳态。关键词:橙子,柠檬烯,固定化,吸附,拉格伦模型
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kinetic Study of limonene and glucose adsorption on immobilization and coimmobilization beads
Rotten oranges contain glucose and limonene, in which limonene is an inhibitor of microorganisms. Immobilization of mixed culture used the entrapment method is the easiest method of protecting the mixed culture from inhibitors. Entrapment method with extrusion drip is an efficient and effective technique to produce beads. This study aims to determine the adsorption rate of adsorbate (glucose and limonene) on the adsorbent surface (beads). Materials used in this study were glucose, DL-limonene, mixed culture, and beads. Three types of beads consisted of alginate - no mixed culture (A), alginate and activated carbon - no mixed culture (CA), alginate and activated carbon - free mixed culture (CB). Adsorption column consist of 30 ml nutrient, 15 mL substrate, and 5 mL beads. If the beads do not contain mixed culture, nutrients and substrate were replaced by aquadest. The reactor was done in a batch system at 37oC. The lowest order of beads ability to adsorb glucose were AG followed by CAG and finally CBG, whereas to limonene solution were AL followed by CBL and finally CAL. Lagergren model was used to determined kinetic bioadsorption on limonene and glucose. The adsorption rate value in the pseudo-second order (k2,ad) for the glucose solution was ranged between 0.025 to 0.087 min-1, while the D-limonene was in the range between 2.084 to 5.233 min-1. Adsorption of glucose and limonene on the surface of the three types of adsorbents was reached steady state at the 60th minute.Keywords: orange, limonene, immobilization, adsorption, Lagergren model.
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