Expression of miR-218 in cervical cancer tissues and bioinformatics analysis of predicted target genes

Objective To investigate the expression of microRNA( miRNA) in cervical cancer tissues and its predicted target genes to provide a theoretical basis for the study on the regulatory mechanism and biological functions of miR-9. Methods MiRNA microarray was applied to detecting the miRNAs expressions in cervical caner tissues and normal cercvical tissues. The biological software was used to predict target genes of up-regulate miRNA-9. The miR-9mimics were transected into cervical cancer cell line Hela,and the mRNA microarrays was used to examine the expression of downstream genes of miR-9. The bioinformatics analysis of the target genes of miR-9 involved gene ontology and signal transduction pathway enrichment. Results Compared with normal cervical tissues,there were 15 miRNAs up-expression and10 miRNAs down-expression in cervical cancer tissues. miR-21 and miR-9 was significantly up-regulated while miR-376a and miR-218 were significantly down-regulated. The mRNA microarrays showed that 148 genes were down expression in miR-9,which also existed in network database,and the genes set mainly located in biosynthetic and metabolic process as well as transcription and protein binding function. The KEGG pathway analysis demonstrated that the gene set mostly located in the regulation of actin cytoskeleton,sphingolipid metabolism and focal adhesion. Conclusion miR-9 expression significantly increases in cervical cancer tissues. The target genes set of miR-9 enriches in multiple biological function and process as well as tumor-related signaling pathways.