培养基组成对几种乳酸菌生物量生长、乳酸和胞外多糖产量的比较影响

B. Adebayo-Tayo, A. Onilude
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引用次数: 7

摘要

为了根据发酵剂的种类和特性来提高发酵乳制品的整体质量,研究了发酵剂组成对产eps乳酸菌的生物量生长、粘度、胞外多糖和乳酸产量的影响。介质组成对研究参数有较大影响。在5种培养基中,部分去蛋白酶乳清培养基(PDW)的生物量增长和黏度产量最大,其中鱼乳球菌(OLHF6)和植物乳杆菌(LPN1)的生物量增长和黏度最高,浓度分别为1.58和1.561mPa.S。植物乳杆菌(Lactobacillus plantarum) (LPN1) (1.58mPa.S)、干酪乳杆菌(LCN1) (1.475 mPa.S)、植物乳杆菌(ln3) (1.48mPa.S)、植物乳杆菌(LPY80) (1.48mPa.S)和鱼乳球菌(OLHF6) (1.185mPa.S)是在各自培养基中形成粘性介质的良好发酵剂。乳清为生产EPS的最佳培养基,干酪乳杆菌(LCN1)为最佳发酵剂(198.69mg/l)。甜乳清培养基、改性MRS培养基、乳清和部分去蛋白乳清培养基均不利于分离菌的乳酸生成,而半清培养基最有利于乳酸生成,其中植物乳杆菌(LPW10)是最佳的乳酸生成发酵剂(5.57g/l)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative influence of medium composition on biomass growth, lactic acid and Exopolysaccharides Production by some Strains of Lactic Acid Bacteria
To promote the overall quality of fermented dairy products (FDP) which depends on the types and characteristics of the starter organisms used in the production, the effect of medium composition on biomass growth, viscosity, exopolysaccharide and lactic acid production by EPSproducing lactic acid bacteria isolates was investigated. Medium composition had profound effect on the studied parameters. Among the five medium used for the cultivation of the isolates, maximum biomass growth and viscosity production was achieved in partially de-protenised whey medium (PDW) in which Lactococcus piscium (OLHF6) and Lactobacillus plantarum (LPN1) exhibit the highest biomass growth and viscosity with concentration of 1.58 and 1.561mPa.S. Lactobacillus plantarum (LPN1) (1.58mPa.S), L.casei (LCN1) (1.475 mPa.S), L.plantarum (LPN3) (1.48mPa.S), L.plantarum (LPY80 (1.48mPa.S) and Lactococcus piscium (OLHF6) (1.185mPa.S) was found to be a good starter for development of viscous medium in the respective media. Whey was the best medium for EPS production in which L. casei (LCN1)) was the best starter (198.69mg/l). Sweet whey medium, modified MRS medium, Whey and partially deproteinised whey medium was not favourable for lactic acid production by the isolates but Semidefine medium was the best for lactic acid development in which L.plantarum (LPW10) was found to be the best starter for lactic acid development (5.57g/l).
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