The Significant Effect of Conditioned Medium of Umbilical Cord Mesenchymal Stem Cells in Histological Improvement of Cartilage Defect in Wistar Rats

Materials and Methods: Twenty-four of 3-month old male rats that were divided into 6 groups (3 control groups and 3 treatment groups) with different evaluation time (month 2, month 3, and month 4). Treatment in each group was repeated 5 times. The cartilage defect was induced manually and mechanically at the medial condyle of rats’ right femur using a Kirschner Wire (D=1.0 mm; h=1.0 mm). The umbilical cord stem cell cultures were obtained from pregnant rats (19 days). Rats in treatment groups were injected with mesenchymal stem cell-conditioned medium (1 mL/kg BW) 5 times with interval a week after cartilage defect. Histopathological examination of chondrocytes formation and fibrosis tissues was done through Haematoxylin Eosin staining. Data were assessed with O’driscoll scoring and analyzed statistically using Kruskal-Wallis test with SPSS 16.0 software statistically using Kruskal-Wallis Test with SPSS 16.0 software.