近红外辐射刺激皮肤伤口修复:可能机制的实验室实验

K. Danno, N. Mori, K. Toda, Takashi Kobayashi, A. Utani
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引用次数: 59

摘要

背景/目的:几种物理药物如低能量激光已被用于慢性皮肤溃疡的治疗。这项研究是为了研究一种新开发的、特定的近红外光源对伤口修复的潜在影响。方法:将培养的人角质形成细胞、内皮细胞和成纤维细胞暴露在光照下,采用酶免疫法、酶谱法和逆转录聚合酶链反应检测转化生长因子(TGF)-β1和基质金属蛋白酶(MMP)-2的产生。在ICR和db/db糖尿病小鼠中建立切口创面,摄影观察辐照对创面愈合的影响。结果:辐照后培养细胞培养液中TGF-β1和MMP-2含量显著升高。从辐照成纤维细胞中提取的MMP-2 mRNA的量也上调。热控制的阴性结果表明,光的作用本质上是热的。在动物模型中,反复暴露可显著加快伤口愈合速度。结论:近红外辐射可能通过其生物刺激作用促进创面愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Near-infrared irradiation stimulates cutaneous wound repair: laboratory experiments on possible mechanisms
Background/Aims: Several physical agents such as low-energy lasers have been used in the treatment of chronic skin ulcers. This study was performed to investigate potential effects of a newly-developed, specific near-infrared light source on wound repair. Methods: Cultured human keratinocytes, endothelial cells and fibroblasts were exposed to the light, and the production of transforming growth factor (TGF)-β1 and matrix metalloproteinase (MMP)-2 was examined by enzyme immunoassay, zymography and reverse transcription polymerase chain reaction. Incisional wounds were created in ICR and db/db diabetic mice and the effect of irradiation on wound closure was followed photographically. Results: The TGF-β1 and MMP-2 content of the medium of cultured cells was significantly elevated after irradiation. The amount of MMP-2 mRNA extracted from irradiated fibroblasts was also upregulated. Negative results in thermal controls suggested that the action of the light was athermic in nature. In animal models, the rate of wound closure was significantly accelerated by repeated exposures. Conclusion: Near-infrared irradiation potentially enhances the wound healing process, presumably by its biostimulatory effects.
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