Marija Mojicevic, J. Grahovac, M. Petkovic, I. Vučković, J. Dodić, S. Dodić, S. Vojnovic
{"title":"土壤分离链霉菌ms1生产尼日利亚菌素和尼菲霉素:抗念珠菌生物测定指导响应面法优化培养mediuМ","authors":"Marija Mojicevic, J. Grahovac, M. Petkovic, I. Vučković, J. Dodić, S. Dodić, S. Vojnovic","doi":"10.2298/fupct1701001m","DOIUrl":null,"url":null,"abstract":"Anti-Candida bioassay guided optimization of the culture medium was used in order to enhance the antifungal activity of the soil isolate MS1. Its morphological, physiological and biochemical characteristics, as well as 16S rDNA sequencing, assigned an MS1 isolate to genus Streptomyces. Optimization of the culture medium was achieved by experimental factorial design and response surface methodology. Maximal antifungal components production was obtained with starch, soybean meal and phosphates content of 40.52, 5.10 and 2.21 g/L, respectively. Chromatography and 1H and 13CNMR spectroscopy were employed for purification and structural characterization of antifungal antibiotics concurrently produced by this strain. These antibiotics were identified as polyether carboxylic antibiotic nigericin and guanidyl-polyol macrolide, niphimycin.","PeriodicalId":12248,"journal":{"name":"Facta Universitatis - Series: Physics, Chemistry and Technology","volume":"38 1","pages":"001-016"},"PeriodicalIF":0.0000,"publicationDate":"2017-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"PRODUCTION OF NIGERICIN AND NIPHIMYCIN BY SOIL ISOLATE STREPTOMYCES SP. MS1: ANTI-CANDIDA BIOASSAY GUIDED RESPONSE SURFACE METHODOLOGY FOR THE OPTIMIZED CULTURE MEDIUМ\",\"authors\":\"Marija Mojicevic, J. Grahovac, M. Petkovic, I. Vučković, J. Dodić, S. Dodić, S. Vojnovic\",\"doi\":\"10.2298/fupct1701001m\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Anti-Candida bioassay guided optimization of the culture medium was used in order to enhance the antifungal activity of the soil isolate MS1. Its morphological, physiological and biochemical characteristics, as well as 16S rDNA sequencing, assigned an MS1 isolate to genus Streptomyces. Optimization of the culture medium was achieved by experimental factorial design and response surface methodology. Maximal antifungal components production was obtained with starch, soybean meal and phosphates content of 40.52, 5.10 and 2.21 g/L, respectively. Chromatography and 1H and 13CNMR spectroscopy were employed for purification and structural characterization of antifungal antibiotics concurrently produced by this strain. These antibiotics were identified as polyether carboxylic antibiotic nigericin and guanidyl-polyol macrolide, niphimycin.\",\"PeriodicalId\":12248,\"journal\":{\"name\":\"Facta Universitatis - Series: Physics, Chemistry and Technology\",\"volume\":\"38 1\",\"pages\":\"001-016\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-12-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Facta Universitatis - Series: Physics, Chemistry and Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2298/fupct1701001m\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Facta Universitatis - Series: Physics, Chemistry and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/fupct1701001m","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
PRODUCTION OF NIGERICIN AND NIPHIMYCIN BY SOIL ISOLATE STREPTOMYCES SP. MS1: ANTI-CANDIDA BIOASSAY GUIDED RESPONSE SURFACE METHODOLOGY FOR THE OPTIMIZED CULTURE MEDIUМ
Anti-Candida bioassay guided optimization of the culture medium was used in order to enhance the antifungal activity of the soil isolate MS1. Its morphological, physiological and biochemical characteristics, as well as 16S rDNA sequencing, assigned an MS1 isolate to genus Streptomyces. Optimization of the culture medium was achieved by experimental factorial design and response surface methodology. Maximal antifungal components production was obtained with starch, soybean meal and phosphates content of 40.52, 5.10 and 2.21 g/L, respectively. Chromatography and 1H and 13CNMR spectroscopy were employed for purification and structural characterization of antifungal antibiotics concurrently produced by this strain. These antibiotics were identified as polyether carboxylic antibiotic nigericin and guanidyl-polyol macrolide, niphimycin.
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