底物多样性影响天然浮游细菌群落的碳利用率和吸收阈值浓度

IF 1.6 4区 环境科学与生态学 Q3 ECOLOGY
J. Sjöstedt, UJ Wünsch, CA Stedmon
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引用次数: 0

摘要

水生环境中溶解有机物(DOM)的持久性可以部分解释为碳底物的高多样性和低浓度。然而,溶解底物质量的变化可以改变水生细菌群落组成和碳吸收速率。本研究的目的是测试多种简单基质的存在是否会影响有机碳的周转。自然细菌群落在连续培养中生长,提供单独的碳底物-水杨酸(SA),色氨酸(Trp)或酪氨酸(Tyr) -或3种底物的组合。使用荧光光谱法追踪浓度,并达到了几纳摩尔的稳态浓度。细菌的生长效率取决于碳源的存在,并在联合处理中达到中间水平。细菌群落保持稳定的色氨酸浓度,在联合处理中比在单独的底物处理中更低。此外,在复合碳基质上生长时,达到稳态浓度的速度更快,但每种化合物的最大利用率较低。然而,联合培养的稳态总碳浓度(SA、色氨酸和Tyr的碳含量之和)高于单独培养基,这似乎是由细菌对最低亲和力的碳基质决定的。本研究结果表明,溶解有机碳的持久性可以部分解释为巨大的底物多样性,这提高了天然细菌群落利用的阈值浓度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Substrate diversity affects carbon utilization rate and threshold concentration for uptake by natural bacterioplankton communities
: Persistence of dissolved organic matter (DOM) in aquatic environments may in part be explained by high diversity and low concentrations of carbon substrates. However, changes in dissolved substrate quality can modify aquatic bacterial community composition and rate of carbon uptake. The aim of this study was to test if the presence of multiple simple substrates affects the turnover of organic carbon. Natural bacterial communities were grown in continuous cultures supplied with either individual carbon substrates — salicylic acid (SA), tryptophan (Trp) or tyrosine (Tyr) — or a combination of the 3 substrates. Concentrations were tracked using fluorescence spectroscopy, and steady-state concentrations of a few nanomolar were reached. Bacterial growth efficiency was dependent on which carbon sources were present and reached an intermediate level in the combined treatment. The bacterial community maintained steady-state concentrations of Trp that were lower in the combined treatment than in the individual substrate treatment. In addition, steady-state concentrations were reached faster during growth on combined carbon substrates, although the maximum utilization rate of each individual compound was lower. However, the steady-state concentration of total carbon (sum of carbon content of SA, Trp and Tyr) was higher in the combined culture than in the individual substrate treatments, and seemed to be determined by the carbon substate for which the bacteria had the lowest affinity. The results from this study indicate that persistence of dissolved organic carbon can in part be explained by vast substrate diversity, which raises the threshold concentration for utilization by natural bacterial communities.
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来源期刊
Aquatic Microbial Ecology
Aquatic Microbial Ecology 环境科学-海洋与淡水生物学
CiteScore
3.30
自引率
0.00%
发文量
8
审稿时长
3.0 months
期刊介绍: AME is international and interdisciplinary. It presents rigorously refereed and carefully selected Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see AME 27:209), Opinion Pieces (previously called ''As I See It'') and AME Specials. For details consult the Guidelines for Authors. Papers may be concerned with: Tolerances and responses of microorganisms to variations in abiotic and biotic components of their environment; microbial life under extreme environmental conditions (climate, temperature, pressure, osmolarity, redox, etc.). Role of aquatic microorganisms in the production, transformation and decomposition of organic matter; flow patterns of energy and matter as these pass through microorganisms; population dynamics; trophic interrelationships; modelling, both theoretical and via computer simulation, of individual microorganisms and microbial populations; biodiversity. Absorption and transformation of inorganic material; synthesis and transformation of organic material (autotrophic and heterotrophic); non-genetic and genetic adaptation; behaviour; molecular microbial ecology; symbioses.
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