脓液细胞作为尼日利亚南南地区GeneXpert MTB/RIF结核分枝杆菌诊断产量的指标:一项前瞻性研究

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
E. Edem, A. Umo, O. J. Akinjogunla, UnwanaEzekiel Akereuke
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引用次数: 1

摘要

背景:结核病(TB)治疗的常见问题是误诊或漏诊导致高发病率和死亡率。为了扭转这种情况,已经开发了检测结核分枝杆菌(MTB)的新诊断工具。然而,在这些工具的评估过程中,许多研究并没有在测试算法中考虑痰液质量的属性。诊断肺结核的实验室检查的效果取决于所测痰样的质量,质量是通过痰中脓和上皮细胞的数量来衡量的。目的和目的:本研究旨在探讨脓液细胞与结核分枝杆菌阳性的关系。方法:采用显微镜、Ziehl-Neelson染色和GeneXpert技术对140例疑似结核病患者的痰液样本进行痰质和结核分枝杆菌筛查。结果:140份痰液标本经AFB镜检和GeneXpert MTB/RIF检测,GeneXpert检测32例(22.9%)MTB阳性,AFB涂片检测27例(19.3%)MTB阳性,痰液类型与AFB产率无显著相关性,而Xpert检测痰液类型与MTB产率有显著相关性(P<0.005)。37份脓细胞≥25的痰液样本中,Xpert检测MTB阳性29份(90.6%),AFB涂片检测MTB阳性25份(92.6%)。结论:总之,我们的研究显示脓细胞的存在与检测方法之间存在高度显著的相关性,因此表明痰中脓细胞的存在可以预测Xpert MTB/RIF对MTB可能的阳性反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pus cell as an indicator for Mycobacterium tuberculosis diagnostic yield by GeneXpert MTB/RIF in South-South Nigeria: A prospective study
Background: The common problem in tuberculosis (TB) management is mis-diagnosis or under diagnosis of cases leading to high morbidity and mortality. In order to reverse this, new diagnostic tools for detection of Mycobacterium tuberculosis (MTB) have been developed. However, in the evaluation process of these tools many studies have not considered attributes of sputum quality in their testing algorithm. The performance of laboratory tests to diagnose pulmonary tuberculosis is dependent on the quality of the sputum sample tested and quality is measured by the number of pus and epithelial cells present in sputum. Aims and Objectives: this study aimed at investigating the association between pus cell and MTB positivity. Methods: Sputum samples from 140 suspected TB participants were screened for sputum quality and MTB using macroscopy, Ziehl-Neelson staining and GeneXpert techniques. Results: Of the 140 sputum samples subjected to AFB microscopy and GeneXpert MTB/RIF assay, GeneXpert assay had 32 (22.9%) MTB positives while AFB smear had 27 (19.3%) MTB positives and there was no significant relationship between sputum type and MTB yield by AFB compared to Xpert that showed significant relationship between sputum type and MTB yield with P<0.005. Out of the 37 sputum samples with pus cells ≥25, 29 (90.6%) were MTB positive by Xpert and 25 (92.6%) were MTB positive by AFB smear. Conclusion: In conclusion, our study shows a high significant association between pus cells presence and the methods and therefore suggests that presence of pus cells in sputum is predictive for possible positivity to MTB by Xpert MTB/RIF.
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来源期刊
Journal of Clinical Sciences
Journal of Clinical Sciences MEDICINE, GENERAL & INTERNAL-
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15
审稿时长
45 weeks
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