DEVELOPMENT AND VALIDATION ZIC HILIC COULOMN FOR SIMULTANEOUS DETERMINATION OF FUROSEMIDE AND INDAPAMIDE AS DOPING

In sports, doping abuse done by athletes has been done since the 18th century. Many of the drugs used for doping and its chemical properties vary. Based on a list issued by the World Anti Doping Agency, one of the doping categories is diuretic. Then the most appropriate testing method is needed to seperation furosemide and indapamide. This study aims to see the development and validation of the separation furosemide and indapamide as doping compounds with High Performance Liquid using the Zic - HILIC column. This study used High Performance Liquid Chromatography of Shidmazu LC 20 AD brand with iso-fed pump and SPD 20A UV-Vis detector. The column used is Zic - HILIC (150 x 4.6 mm; 5 μm). This research is carried out on the optimization of conditions including mobile phase velocity, mobile phase pH, buffer of mobile phase, and buffer concentration. Selection of selected conditions see the price of R> 1.5 and N >>> or HETP << 1.5 and the N price is the greatest. The method was than validated forSelectivity, linearity,accuracy, precision, limits ofdetection and limits ofquantification. The calibration curve of furosemide and indapamide were linear with a regression coefficient R > 0.999.