{"title":"用酿酒酵母载体RDT法从山羊胰腺中分离、制备、纯化、测定和鉴定胰岛素","authors":"","doi":"10.46243/jst.2020.v5.i6.pp107-110","DOIUrl":null,"url":null,"abstract":"Insulin is a polypeptide hormone secreted by the β-cell of Islet’s of langerhan’s of the pancreas and\ncosists of two polypeptide chain-A and chain- B. It is linked by two inter chain disulphide bonds (A7- B7 and A20-B19)\nand also has an intra-chain disulphide bond in the A-Chain (A6-A11). For production of Insulin from goat pancreas\nsaccharomyces cerevisae using as a vector. In this study Recombinant DNA technology, chromatography,\nElecrophoresis, Spectrophotometer, SDS-PAGE, zymography etc techaniques were used. Humulin was tanken as a\nmarker. It was found, goat insulin showed good result. 5.8 – 6.5 KDa recombinant insulin was calculated. It did not\nshow antigenic proterties.","PeriodicalId":23534,"journal":{"name":"Volume 5, Issue 4","volume":"122 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation, Production, Purification, Assay and Characterization of\\nInsulin From Goat (Capra Hircus) Pancreas By RDT Using\\nSaccharomyces Cerevisae Vector\",\"authors\":\"\",\"doi\":\"10.46243/jst.2020.v5.i6.pp107-110\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Insulin is a polypeptide hormone secreted by the β-cell of Islet’s of langerhan’s of the pancreas and\\ncosists of two polypeptide chain-A and chain- B. It is linked by two inter chain disulphide bonds (A7- B7 and A20-B19)\\nand also has an intra-chain disulphide bond in the A-Chain (A6-A11). For production of Insulin from goat pancreas\\nsaccharomyces cerevisae using as a vector. In this study Recombinant DNA technology, chromatography,\\nElecrophoresis, Spectrophotometer, SDS-PAGE, zymography etc techaniques were used. Humulin was tanken as a\\nmarker. It was found, goat insulin showed good result. 5.8 – 6.5 KDa recombinant insulin was calculated. It did not\\nshow antigenic proterties.\",\"PeriodicalId\":23534,\"journal\":{\"name\":\"Volume 5, Issue 4\",\"volume\":\"122 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-10-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Volume 5, Issue 4\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.46243/jst.2020.v5.i6.pp107-110\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Volume 5, Issue 4","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46243/jst.2020.v5.i6.pp107-110","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation, Production, Purification, Assay and Characterization of
Insulin From Goat (Capra Hircus) Pancreas By RDT Using
Saccharomyces Cerevisae Vector
Insulin is a polypeptide hormone secreted by the β-cell of Islet’s of langerhan’s of the pancreas and
cosists of two polypeptide chain-A and chain- B. It is linked by two inter chain disulphide bonds (A7- B7 and A20-B19)
and also has an intra-chain disulphide bond in the A-Chain (A6-A11). For production of Insulin from goat pancreas
saccharomyces cerevisae using as a vector. In this study Recombinant DNA technology, chromatography,
Elecrophoresis, Spectrophotometer, SDS-PAGE, zymography etc techaniques were used. Humulin was tanken as a
marker. It was found, goat insulin showed good result. 5.8 – 6.5 KDa recombinant insulin was calculated. It did not
show antigenic proterties.
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