聚多巴胺在多用途表面上的通用打印技术,用于使用湿弹性邮票进行高分辨率细胞图案

Woojin Chae, N. Lee
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引用次数: 0

摘要

在这项研究中,一种强大且普遍适用的聚多巴胺(PDA)接触印刷技术被证明是在多种基材上,如玻璃、聚对苯二甲酸乙二醇酯、聚(甲基丙烯酸甲酯)、聚苯乙烯、聚碳酸酯、铜和硝化纤维素膜上,以一种简单易用的方式使用湿弹性体印章。在与基材接触时,湿图章上的水化层的蒸发大大提高了图案化效率,甚至无需在图章上施加任何重量。水化层可能有助于减少在处理印章期间引起的机械应力,并在干燥时增强印章和基材之间的保形接触。当在聚苯乙烯上进行图案制作时,PDA的图案效率比使用干式印章高出约五倍,并且还实现了直径超过8.5厘米的大规模PDA冲压。水接触角测量和傅里叶变换红外光谱(FTIR)分析证实了PDA在各种表面上的成功转移。在聚苯乙烯上创建的PDA模式用于培养内皮细胞,以评估沿定义几何形状扩散的空间定义细胞。所使用的底物的简单程序和多功能性使所介绍的策略非常适合于创建大规模细胞微图谱平台,并且具有制造抗体固定化横向流动快速诊断试剂盒的巨大潜力,而不需要昂贵的设备。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Universal Printing Technique of Polydopamine onto Versatile Surfaces for High‐Resolution Cell Patterning Using Wet Elastomeric Stamp
In this study, a robust and universally applicable polydopamine (PDA) contact‐printing technique is demonstrated on versatile substrates such as glass, polyethylene terephthalate, poly(methyl methacrylate), polystyrene, polycarbonate, copper, and nitrocellulose membrane in a simple and facile manner using a wet elastomeric stamp. Evaporation of the hydration layer on the wet stamp while in contact with substrates substantially increases the patterning efficiency even without placing any weight on the stamp. The hydration layer possibly assists in reducing the mechanical stress caused during the handling of the stamp and enhances the conformal contact between the stamp and the substrate upon drying. The PDA patterning efficiency is approximately fivefold higher compared to using a dry stamp when patterned on polystyrene, and a large‐scale PDA stamping of over 8.5 cm diameter is also achieved. Water contact angle measurements and Fourier‐transform infrared spectroscopy (FTIR) analyses confirms the successful transfer of PDA onto various surfaces. PDA patterns created on the polystyrene are used to culture endothelial cells to evaluate spatially‐defined cell spreading along the defined geometries. The simple procedure and versatility of the substrates used make the introduced strategy highly suitable for creating large‐scale cell micropatterning platforms and possess great potential for manufacturing antibody‐immobilized lateral flow rapid diagnostic kits, without requiring expensive equipment.
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